Pre-Test Discussion

Question 1

It has uniform and thin walls which enables a precise thermal transfer

Answer 1

96 Well PCR Plates

Question 2

A thermocycler or PCR machine used to amplify segments of DNA via Polymerase chain reaction

Answer 2

Biorad CFX 96

Question 3

Two parts of Biorad CFX 96

Answer 3

Annealing chamber thermal rotation and a Real-time device

Question 4

It provides a table platform for PCR Plates, strips and reagents

Answer 4

Cold Block

Question 5

Used to spin down samples at high speed

Answer 5

Microcentrifue

Question 6

Singe use tubes made of polypropylene used for preparing, mixing, centrifuging, transporting and storing small volume of liquid samples

Answer 6

Microcentrifuge tubes

Question 7

Microcentrifuge tubes are made up of

Answer 7

Polypropylene

Question 8

It is a ventilated enclosure that ensures product protection only

Answer 8

PCR Cabinet

Question 9

Explain how is PCR Cabinet provides product protection ONLY

Answer 9

Air coming from the outside is being filtered before entering the cabinet but exhaust air is not filtered, thus providing product protection only.

Question 10

Used to spin down all droplets on the wall of PCR Plates

Answer 10

PCR Plate Centrifuge

Question 11

Detect the presence of SARS-CoV 2 RNA by amplifying a target gene

Answer 11

SARS-CoV 2 Detection Kit

Question 12

SARS-CoV 2 Detection Kit consists of

Answer 12

Enzymes, Probes, dNTPs, and a buffer

Question 13

Used in solid-phase extraction method

Answer 13

Spin column

Question 14

It is used to mix volume sample and reagent rapidly

Answer 14

Vortex Mixer

Question 15

What enzymes do SARS-CoV 2 Detection Kit consists?

Answer 15

Taq DNA Polymerase and Reverse transcriptase