Molecular Laboratory Introduction

Question 1

Services offered in VMMC Molecular Laboratory

Answer 1

SARS-CoV-2- Reverse Transcription

Polymerase Chain Reaction (RT-PCR)

Question 2

COVID-19 Testing Laboratory was built in the year

Answer 2

2020

Question 3

Three phases of Laboratory Testing

Answer 3

Pre-Analytical, Analytical, & Post-analytical

Question 4

The first and crucial step in laboratory testing

Answer 4

Specimen collection

Question 5

What are the different specimen collection materials?

Answer 5

Virus Transport Media
Swabs
Parafilm, Gauze, Triple Package
Case investigation Form

Question 6

All except one is included in the label of VTM
Full Name
Birthday
Age/Gender
Data of Collection
Time of Collection
Sample Collected
Swabber's Initial

Answer 6

Swabber’s Initial

Question 7

Transcribe OPS & NPS

Answer 7

Oropharyngeal swab

Nasopharyngeal swab

Question 8

TRUE OR FALSE:

Always check the VTM’s volume (should be at least 3mL or depending on manufacturer’s guide)

Answer 8

FALSE

At least 1mL or depending on manufacturer’s guide

Question 9

TRUE OR FALSE:

Always check on the VTM’s expiration date before using

Answer 9

True

Question 10

TRUE OR FALSE:

Nasopharyngeal swab is thicker than the Oropharyngeal swab

Answer 10

False

Oropharyngeal swab is thicker

Question 11

What type of swab are unacceptable in the collection of specimen for SARS-COV-2 detection? Why?

Answer 11

Calcium alginate swabs or swabs with wooden shaft because they may contain substances that inactivate viruses and inhibits PCR Testing

Question 12

Handling of swab samples follows what rule?

Answer 12

Triple Packaging System

Question 13

It is used to prevent leakage for screw cap of VTM

Answer 13

Parafilm

Question 14

It is used to absorb any form of leakage coming form the VTM

Answer 14

Gauze

Question 15

It is used to prevent sample spills and contamination to other VTMs

Answer 15

Primary container

Question 16

It is used to prevent sample spills and maintain specimen stability

Answer 16

Secondary container

Question 17

It is used for transporting VTMs to the molecular laboratory

Answer 17

Tertiary container

Question 18

True or False:

NO CIF, NO SWABBING

Answer 18

True

Question 19

PAGE 1 has 2 parts, namely;

Answer 19

Patient information and Case investigation details

Question 20

Identify the following categories:
RPV
Vmmcp
CP
RPVD, CMMCPD

Answer 20

RPV: Retired veteran
Vmmcp: Vmmc employee
CP: Civilian patients
RPVD, CMMCPD: Corresponding dependents

Question 21

CIF consists of how many pages?

Answer 21

3

Question 22

TRUE OR FALSE:
Page 2 consists of clinical information and to be signed by the physician
Page 3 entails case definition

Answer 22

TRUE

Question 23

What are the ideal sites for specimen collection for the test for COVID-19

Answer 23

NPS and OPS

Question 24

What temperature is required for the storage of VTM with swab?

Answer 24

2-8 °C (short term storage)
freezer temperature (long term storage)

Question 25

Tertiary container is labelled as __?

Answer 25

Biohazard

Question 26

Specimens should be transported within _____?

Answer 26

72 hours or less