SF I Lab: Gram Neg Flashcards
Used primarily to differentiate members of Enterobacteriaceae family from other GN rods
Triple Sugar Iron
Indicator of Hydrogen Sulfide
Ferric Ammonium Citrate
Expected Result: lactose and glucose fermenter
A/A (Acid slant/ Acid Butt)
Reporting for Non lactose fermenter but glucose fermenter and H2s positive
K/A H2s+
How many hours do we incubate the TSI tube in ambient air?
18-24 hours
Ph indicator for TSI
Phenol red
Used to differentiate Gram negative bacteria on the deaminatio of phenylalanine
Phenylalanine Deaminase Agar
Reagent for Phenylalanine Deaminase Agar
10% Ferric Chloride (FeCl3)
Product for Phenylalanine Deaminase Agar
Green Color
How many drop(s) of 24 hour BHI broth do we add to inoculate phenylalanine?
1 drop
Used to differentiatw Gram negative bacilli based on the decarboxylation or deaminatio of lysine and H2s production
Lysine Iron Agar
pH Indicator for LIA
Bromcresol purple
Amino acid source for LIA
L-lysine
Product for Lysine Deamination
Burgundy/ Bordeaux Red Color
Enzyme for LIA
Lysine Deaminase
Substrate for LIA
Lysine
Lysine decarboxylation positive reaction
Purple Color
Product for Lysine Decarboxylation
CO2 + Cadaverine
Deaminase negative color
Purple
Decarboxylase negative color
Yellow
Detects the presence of tryptophanase
Indole Test
Reagent use for indole test
dimethylamine-benzaldehyde
Product for Indole test
Triarylmethane Dye (Red)
Rapid/Spot indole test reagent
Paradimethylamino-cinnamaldehyde
Detects the presence of glucose metabolic by products via the mixed acid fermentation pathway
Methyl Red Test
Number of methyl red drops use per 5 ml of Methyl red broth
5 or 6
Detects thw presence of glucose metabolic by products via butylene glycol pathway
Voges-Proskauer Test
Reagent use for Voges-Proskauer Test
Potassium hydroxise (KOH) & a-naphthol
Number of drops of alpha-naphthol use for VP broth
6 drops
Number of minutes VP broth should be incubated for the second time after shaking the tube
30 minutes
Determines whether an organism can utilize citrate as the sole carbon source and inorganic ammonium salta as the sole nitrogen source
Citrate Utilization
pH indicator of Citrate utilization test
Bromthymol blue
Positive reaction of Citrate utilization test
Prussian blue
Negative reaction for Citrate utilization test
Green
This test is used to determine the ability of an organism to reduce nitrate to nitrite
Nitrate reduction
True negative reaction of Nitrate reduction test
Nitrate + Zinc powder = Nitrate ( reacts with reacts with reagent to form red dye)
Reagent for Nitrate reduction
Sulfanilic acid + alpha-naphthylamine
False negative reaction of Nitrate reduction
Organisms reduced it further to NH3, NO, or N2O2
Tube use for nitrate reduction test to identify gas production
Durham tube
Substrate for ONPG test
O-nitrophenyl-b-D-Galactopyranoside
ONPG test product
Ortho-nitrophenol
Positive reaction for ONPG test
Yellow
Test is used to determine an organism’s ability to produce the enzyme urease, which hydrolyzes urea
Urease Test (Christensen’s Method)
Positive reaction for urease test
Magenta
