Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Bacteriology Lecture (Midterm- Finals) > SF I Lab: Gram Neg > Flashcards

SF I Lab: Gram Neg Flashcards

1
Q

Used primarily to differentiate members of Enterobacteriaceae family from other GN rods

A

Triple Sugar Iron

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Indicator of Hydrogen Sulfide

A

Ferric Ammonium Citrate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Expected Result: lactose and glucose fermenter

A

A/A (Acid slant/ Acid Butt)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Reporting for Non lactose fermenter but glucose fermenter and H2s positive

A

K/A H2s+

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

How many hours do we incubate the TSI tube in ambient air?

A

18-24 hours

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Ph indicator for TSI

A

Phenol red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Used to differentiate Gram negative bacteria on the deaminatio of phenylalanine

A

Phenylalanine Deaminase Agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Reagent for Phenylalanine Deaminase Agar

A

10% Ferric Chloride (FeCl3)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Product for Phenylalanine Deaminase Agar

A

Green Color

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

How many drop(s) of 24 hour BHI broth do we add to inoculate phenylalanine?

A

1 drop

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Used to differentiatw Gram negative bacilli based on the decarboxylation or deaminatio of lysine and H2s production

A

Lysine Iron Agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

pH Indicator for LIA

A

Bromcresol purple

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Amino acid source for LIA

A

L-lysine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Product for Lysine Deamination

A

Burgundy/ Bordeaux Red Color

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Enzyme for LIA

A

Lysine Deaminase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Substrate for LIA

A

Lysine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Lysine decarboxylation positive reaction

A

Purple Color

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Product for Lysine Decarboxylation

A

CO2 + Cadaverine

19
Q

Deaminase negative color

A

Purple

20
Q

Decarboxylase negative color

A

Yellow

21
Q

Detects the presence of tryptophanase

A

Indole Test

22
Q

Reagent use for indole test

A

dimethylamine-benzaldehyde

23
Q

Product for Indole test

A

Triarylmethane Dye (Red)

24
Q

Rapid/Spot indole test reagent

A

Paradimethylamino-cinnamaldehyde

25
Q

Detects the presence of glucose metabolic by products via the mixed acid fermentation pathway

A

Methyl Red Test

26
Q

Number of methyl red drops use per 5 ml of Methyl red broth

A

5 or 6

27
Q

Detects thw presence of glucose metabolic by products via butylene glycol pathway

A

Voges-Proskauer Test

28
Q

Reagent use for Voges-Proskauer Test

A

Potassium hydroxise (KOH) & a-naphthol

29
Q

Number of drops of alpha-naphthol use for VP broth

A

6 drops

30
Q

Number of minutes VP broth should be incubated for the second time after shaking the tube

A

30 minutes

31
Q

Determines whether an organism can utilize citrate as the sole carbon source and inorganic ammonium salta as the sole nitrogen source

A

Citrate Utilization

32
Q

pH indicator of Citrate utilization test

A

Bromthymol blue

33
Q

Positive reaction of Citrate utilization test

A

Prussian blue

34
Q

Negative reaction for Citrate utilization test

A

Green

35
Q

This test is used to determine the ability of an organism to reduce nitrate to nitrite

A

Nitrate reduction

36
Q

True negative reaction of Nitrate reduction test

A

Nitrate + Zinc powder = Nitrate ( reacts with reacts with reagent to form red dye)

37
Q

Reagent for Nitrate reduction

A

Sulfanilic acid + alpha-naphthylamine

38
Q

False negative reaction of Nitrate reduction

A

Organisms reduced it further to NH3, NO, or N2O2

39
Q

Tube use for nitrate reduction test to identify gas production

A

Durham tube

40
Q

Substrate for ONPG test

A

O-nitrophenyl-b-D-Galactopyranoside

41
Q

ONPG test product

A

Ortho-nitrophenol

42
Q

Positive reaction for ONPG test

A

Yellow

43
Q

Test is used to determine an organism’s ability to produce the enzyme urease, which hydrolyzes urea

A

Urease Test (Christensen’s Method)

44
Q

Positive reaction for urease test

A

Magenta

Bacteriology Lecture (Midterm- Finals) (9 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions