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Bacteriology Lecture (Midterm- Finals) > MIDTERM: Mycobacteria > Flashcards

MIDTERM: Mycobacteria Flashcards

1
Q

Classify the Runyon group: M.thermoresistible

A

Scotochromogens

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2
Q

measures the presence of a lipase.  Hydrolysis causes a pink color change

A

Tween 80 hydrolysis

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3
Q

Exposed to direct sunlight, MTB for culture are killed within

A

within 2 hours

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4
Q

grow on chocolate agar and appear as smooth. opaque colonies resembling staphylococci or yeast.

A

M. chelonae

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5
Q

Contains 0.1% enzymatic hydrolysate of casein, which improves recovery of isoniazid-resistant Mycobacterium tuberculosis

A

Middlebrook 7H11

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6
Q

positive control organism for urease

A

M. fortuitum

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7
Q

Test that utilize M.intracellulare as negative control

A

Niacin,Nitrate, Tween 80, Arylsulfatase (Naya and Nit twerk around Ariel during INTRAmurals)

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8
Q
A
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9
Q

does not reduce nitrates or assimilate iron, but it is suceptible to polymyxin B and resistant to ciprofloxacin.

A

M. chelonae

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10
Q

have the ability to grow at 45°C; does not grow well on LJ agar. It appears as a film after 3 to
4 weeks of incubation

A

M. avium

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11
Q

Classify the Runyon group: M.smegmatis

A

Rapid growers

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12
Q

Supplemented with cycloheximide,
lincomycin, and nalidixic acid

A

L-J Mycobactosel

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13
Q

Not pigmented unless exposed to light optimally during their early growth

A

Photochromogen

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14
Q

span of growth in liquid media

A

10 days

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15
Q

appear as a “sun-spot” with starlike or asteroid margins; rough colony has a “lacy” characteristic

A

M. avium

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16
Q

Test that utilize M.gordonae as negative control

A

5% NaCl (Chicken GORDON with salt(NaCl)

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17
Q

Pigmented when grown in the dark or light

A

Scotochromogen

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18
Q

Battey Bacillus
 In AIDS patients

A

MYCOBACTERIUM AVIUM COMPLEX

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19
Q

Negative result reaction in Niacin Test

A

M. intracellulare-No color change (milky white)

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20
Q

a disseminated infection with multiple organ
involvement.

A

Miliary TB

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21
Q

Decontaminating agent of MYCOBACTERIUM LEPRAE

A

N acetyl-L-cysteine(NALC)

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22
Q

MYCOBACTERIUM TUBERCULOSIS measurement

A

0.2-0.6 micron in diameter and 1-4 micra in length

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23
Q

Test that utilize M.tuberculosis as negative control

A

68C catalase, Tellurite, TCH (TCH & Tell got 68 on TB test)

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24
Q
A
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25
Q

liquid media that is used as the standard for Isolating mycobacteria

A

Löwenstein Jensen (L-J)

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26
Q

M.xenopi runyon’s class

A

Scotochromogen

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27
Q

a widely used medium for the isolation and antimicrobial susceptibility testing of mycobacteria

A

Middlebrook 7H11 agar

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28
Q

Droplets of dried sputum in the air -

A

8-10 days

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29
Q

3-week-old colony growing on Middlebrook 7H11agar shows a dark, dense center and a wrinkled periphery,

A

Colony of M. kansasii

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30
Q

pyrazinamide is converted into pyrazinoic acid through the action of the pyrazinamidase enzyme

A

Pyrazinamide test

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31
Q

Not pigmented when grown in the dark or in light

A

Nonchromagen

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32
Q

have a strong yellow color; a positive catalase reaction, reduction of nitrate to nitrite, and rapid hydrolysis of Tween 80

A

M. kansasii on Middlebrook 7H11 agar.

33
Q

detected by adding phenolphthalein to thec olony/ substrate mixture and observing the formation of a pink color

A

Arylsulfatase test

34
Q

Span of MTB In sputum -

A

20-30 hours

35
Q

disseminated disease in HIV-infected patients

A

MYCOBACTERIUM GENAVENSE

36
Q

etiologic agent of leprosy, also called Hansen’s disease

A

MYCOBACTERIUM LEPRAE

37
Q

rough, buffered colonies that appeared within 3 weeks

A

M.tuberculosis in LJ agar

38
Q

Mycobacteria that are thermolabile in catalase test

A

M. tuberculosis and M. bovis

39
Q

Disinfection of sputum -

A

24 hours in 5% phenol

40
Q

Positive reaction for catalase test

41
Q

positive control organism for Tellurite test

42
Q

Optimum temperature for M. fortuitum cultured in 7H11 & LJ

A

43 deg. Celsius

43
Q

Contains 2% glycerol, which enhances the growth of Mycobacterium avium complex (MAC)

A

Middlebrook

44
Q

Highly resistant to drying (protected from sunlight)

A

MYCOBACTERIUM TUBERCULOSIS

45
Q

Bairnsdale ulcer

A

MYCOBACTERIUM ULCERANS

46
Q

MTB antigen.

A

purified protein derivative (PPD)

47
Q

positive control organism for TCH

48
Q

Growth at 28°C on an egg based medium containing 5% NaCl

A

NaCl tolerance test

49
Q

not culturable; unique manifestation in a way that they infect the nerves instead the cells

A

MYCOBACTERIUM LEPRAE

50
Q

a Runyon group II scotochromogens, characterized by yellow-orange pigment

A

M. gordonae

51
Q

Reagent strip impregnated with substrate cyanogen halide = yellow color

A

Niacin accumulation tests

52
Q

Classify the Runyon group: M.terrae-triviale

A

Non-photochromogens

53
Q

Nontuberculous mycobacteria pigment

A

Runyon’s classification

54
Q

Supplemented with penicillin and nalidixic acid

55
Q

Duration of MTB in Dried sputum

A

6-8 months

56
Q

Colonies appear as cream-colored, dry, and wrinkled.

A

M. tuberculosis on Middlebrook 7H11 agar

57
Q

colonies appear dark and dense with smooth edges and a somewhat lighter center

A

M. chelonae

58
Q

digesting agent MYCOBACTERIUM LEPRAE

59
Q

The only mycobacteria that is negative to NaCl Tolerance test

A

M.chelonae

60
Q

Tap-water bacillus

A

MYCOBACTERIUM GORDONAE

61
Q

M.kansassi runyon’s classification

A

Photochromogen

62
Q

Contains twice the concentration
of malachite than LöwensteinJensen green

A

Petragnani medium

63
Q

growth requirement for hemoglobinor hemin

A

MYCOBACTERIUM HAEMOPHILUM

64
Q

contains casein hydrolysates that improve the recoveryof isoniazid-resistant strains of M. tuberculosis and shortens the incubation time for M. avium complex isolates.

A

Middlebrook 7H11 agar

65
Q

is miliary tuberculosis in the bones or spine

A

Pott’s disease

66
Q

Supplemented with carbenicillin
(for inhibition of pseudomonads),
polymyxin B, trimethoprim
lactate, and amphotericin B

A

Middlebrook 7H10

67
Q

positive for Arylsulfatase test

A

M. fortuitum and M. abcesuss;

68
Q

chronic granulomatous infections involving skin and soft tissue, often called”swimming pool granuloma” or “fish tank granuloma”

A

MYCOBACTERIUM MARINUM

69
Q

Commonly used medium; good
recovery of M. tuberculosis
but poor recovery of many other species; M. genovense fails to grow

A

Löwenstein Jensen (L-J)

70
Q

positive control organism for Tween 80 hydrolysis

A

M.kansasii

71
Q

used to detect MTB-infected individuals

A

Tuberculin skin test

72
Q

can also grow on modifiedMac-Conkey agar, without crystal violet, at 37°C;

A

M. fortuitum

74
Q

“yellow bacillus,

A

MYCOBACTERIUM KANSASII

75
Q

colonies have a rough appearance and exhibit serpentine cording,exemplified by the darker areas.

A

M. tuberculosis

76
Q

positive control organism for Niacin test

A

M.tuberculosis

77
Q

Supplemented with hemin, hemoglobin, or ferric ammonium citrate; increases recovery of
Mycobacterium haemophilum

A

Heme-supplemented
media (egg or agar
based)

78
Q

Base on the ability to convert ferric ammonium citrate to an iron oxide

A

Iron uptake test

79
Q

may appear as weakly staining. beaded, gram-positive bacilli, suggesting the possibility of a rapidly growing mycobacterium.

A

M. chelonae

Bacteriology Lecture (Midterm- Finals) (9 decks)

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