MIDTERM: Mycobacteria Flashcards
Classify the Runyon group: M.thermoresistible
Scotochromogens
measures the presence of a lipase. Hydrolysis causes a pink color change
Tween 80 hydrolysis
Exposed to direct sunlight, MTB for culture are killed within
within 2 hours
grow on chocolate agar and appear as smooth. opaque colonies resembling staphylococci or yeast.
M. chelonae
Contains 0.1% enzymatic hydrolysate of casein, which improves recovery of isoniazid-resistant Mycobacterium tuberculosis
Middlebrook 7H11
positive control organism for urease
M. fortuitum
Test that utilize M.intracellulare as negative control
Niacin,Nitrate, Tween 80, Arylsulfatase (Naya and Nit twerk around Ariel during INTRAmurals)
does not reduce nitrates or assimilate iron, but it is suceptible to polymyxin B and resistant to ciprofloxacin.
M. chelonae
have the ability to grow at 45°C; does not grow well on LJ agar. It appears as a film after 3 to
4 weeks of incubation
M. avium
Classify the Runyon group: M.smegmatis
Rapid growers
Supplemented with cycloheximide,
lincomycin, and nalidixic acid
L-J Mycobactosel
Not pigmented unless exposed to light optimally during their early growth
Photochromogen
span of growth in liquid media
10 days
appear as a “sun-spot” with starlike or asteroid margins; rough colony has a “lacy” characteristic
M. avium
Test that utilize M.gordonae as negative control
5% NaCl (Chicken GORDON with salt(NaCl)
Pigmented when grown in the dark or light
Scotochromogen
Battey Bacillus
In AIDS patients
MYCOBACTERIUM AVIUM COMPLEX
Negative result reaction in Niacin Test
M. intracellulare-No color change (milky white)
a disseminated infection with multiple organ
involvement.
Miliary TB
Decontaminating agent of MYCOBACTERIUM LEPRAE
N acetyl-L-cysteine(NALC)
MYCOBACTERIUM TUBERCULOSIS measurement
0.2-0.6 micron in diameter and 1-4 micra in length
Test that utilize M.tuberculosis as negative control
68C catalase, Tellurite, TCH (TCH & Tell got 68 on TB test)
liquid media that is used as the standard for Isolating mycobacteria
Löwenstein Jensen (L-J)
M.xenopi runyon’s class
Scotochromogen
a widely used medium for the isolation and antimicrobial susceptibility testing of mycobacteria
Middlebrook 7H11 agar
Droplets of dried sputum in the air -
8-10 days
3-week-old colony growing on Middlebrook 7H11agar shows a dark, dense center and a wrinkled periphery,
Colony of M. kansasii
pyrazinamide is converted into pyrazinoic acid through the action of the pyrazinamidase enzyme
Pyrazinamide test
Not pigmented when grown in the dark or in light
Nonchromagen