Sexual Assault Evidence Flashcards

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1
Q

Seminal Plasma

A
Composed of 
Proteins
Salts
Sugars
Lipids
Enzymes (Acid phosphatase)
Nutrients 
Hormones
Basic amines (spermine)
P30 (prostate specific antigen-PSA)
Choline 
Cellular material
Flavins (source of flourescence under UV -seen using ALS)
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2
Q

Fluid delivered through penis in ejaculation

A

Semen

*spermatozoa (cellular component) = plural

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3
Q

Acid phosphatase (AP) and Prostate Specific Antigen (PSA) both come from ______

A

Prostate gland

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4
Q

Seminiferous tubles

A

Long thread like tubes found in each of the testicles where spermatozoa are produced

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5
Q

Coagulation

A

Putting together making gel like substance

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6
Q

Seminal plasma secretions

A
Testes
Epididymides
Seminal vesicles 
Prostate
Cowper's glands
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7
Q

Spermatozoon

A

Singular

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8
Q

Half sperm = X

Half sperm = Y

A

Haploid cell

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9
Q

Morphology of sperm

A

Head -contains nucleus & acrosome

Tail and Midpiece- contain mechanisms and enzymatic materials for motility

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10
Q
  • Contains mitochondria
  • Each sperm mitochondrion carries multiple copies of paternal mitochondria genome
  • energy for sperm flagellar motility
  • joins head and tail
A

Midpiece (neck)

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11
Q

Mobility or motility of spermatozoa

A

Tail

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12
Q

NFSTC

A

National forensic science technology center

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13
Q

Protamines

A

Arginine rich nuclear protein that replaces histones late in haploid phase of soermatogenesis

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14
Q

Two types of protamines are______

A

P1 & P2

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15
Q

Aspermia

A

No ejaculation (absence of spermatozoa)

  • infertility
  • drug use
  • prostate surgery
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16
Q

Azoospermia

A

Lack of sperm cells in the semen (no spermatozoa)

  • temporary or permanent
  • vasectomy
  • drugs
  • alcoholism
  • high fever
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17
Q

Oligospermia (asthenozoospermia)

A

Fewer number of spermatozoa

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18
Q

Teratozoospermia

A

Increased abnormal forms of sperm

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19
Q

Oligoasthenoteratozoospermia

A

Spermatozoa variables all subnormal (OAT)

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20
Q

Leucocytospermia

A

Increased leukocytes in semen

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21
Q

Necrozoospermia

A

All sperm non viable or non motile

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22
Q

Globozoospermia

A

Sperm heads lack acrosome caps and can’t fertilize

rare*

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23
Q

Phagocytic

A

Eats other cells

24
Q

Protease

A

Break up of proteins

25
Q

high concentration in semen and liquefies seminal fluid

  • serine protease
  • produced by epithelial cells & found in seminal fluid
A

PSA

26
Q

Protease

A

Break up of proteins

27
Q

high concentration in semen and liquefies seminal fluid

  • serine protease
  • produced by epithelial cells & found in seminal fluid
A

PSA

28
Q

Protease

A

Break up of proteins

29
Q

high concentration in semen and liquefies seminal fluid

  • serine protease
  • produced by epithelial cells & found in seminal fluid
A

PSA

30
Q

Y shaped proteins

A

Immunoglobulin G (IgG)

31
Q

ELISA

A

Enzyme linked immunosorbent assay

32
Q

RSID

A

Rapid Stain Identification Semen Test

33
Q

ABAcard P30 detects_____

A

PSA

34
Q

RSID detects ______

A

Semenogelin (Sg)

35
Q

3 major gel-forming proteins

A

Fibronectin
Semenogelin I
Semenogelin II

36
Q

Semenogelin I

A

Disulfide linked complex of ~52-kDa protein

37
Q

Semenogelin II

A

Two forms of an Sg I related protein of ~71 and ~76 kDa

38
Q

Seminiferous tubules

A

Where sperm is being produced

39
Q

Epididymis

A

A coiled tube next to each testis and receives sperm from the seminiferous tubules

40
Q

Three phases of spermatogenesis

A

Spermatocytogenesis
Diploid cell meiosis
Spermiogenesis

41
Q

Spermatocytogensis

A

Immature spermatogonia undergo mitotic division

*where mitosis takes place

42
Q

Diploid cells -Meiosis

A

Diploid number present in first phase becomes haploid, 23 chromosomes

*secondary spermatocytes (haploid spermatids)

43
Q

Spermiogenesis

A

Spermatids undergo morphological changes

*final phase-spermatids are haploid, see acrosome form & elongated

44
Q

Spermiogenesis

A

Midpiece - mitochondria

Acrosome - enzymatic knife used to penetrate the oocyte

Nucleus - haploid chromosomes

45
Q

Vas deferens

A
  • Main vesicle for carrying sperm
  • travels out scrotum–> into abdomen–> & passes behind urinary bladder–> expands to form expanded end part (ampulla)

Ampulla =bulb shape

46
Q

Urethra

A

Carries semen (sperm) through penis during intercourse and during urination passes through it

*two different functions, do not occur at the same time

47
Q

SRY

A

Sex determining region

*Y is smaller then X

48
Q

ZFX

A

Zinc fingerprint protein

  • located on X chromosome
  • extremely important in humans
49
Q

Danes method

A

Keratin
Prekeratin
Mucin

50
Q

Ayoub-Shklar

A

Differentiate skin epithelial cells from buccal and vaginal

51
Q

VAP

A

Vaginal acid phosphatase

52
Q

SAP

A

Seminal acid phosphatase

53
Q

ELISA

A

Enzyme linked immunosorbent assay

54
Q

KPIC

A

Kernechtrot Picroindigocarmine stain

55
Q

3 methods Fertility and Sterility

A

Shorr
Papanicolau (papsmear)
Testsimplets