Sexual Assault Evidence Flashcards
Seminal Plasma
Composed of Proteins Salts Sugars Lipids Enzymes (Acid phosphatase) Nutrients Hormones Basic amines (spermine) P30 (prostate specific antigen-PSA) Choline Cellular material Flavins (source of flourescence under UV -seen using ALS)
Fluid delivered through penis in ejaculation
Semen
*spermatozoa (cellular component) = plural
Acid phosphatase (AP) and Prostate Specific Antigen (PSA) both come from ______
Prostate gland
Seminiferous tubles
Long thread like tubes found in each of the testicles where spermatozoa are produced
Coagulation
Putting together making gel like substance
Seminal plasma secretions
Testes Epididymides Seminal vesicles Prostate Cowper's glands
Spermatozoon
Singular
Half sperm = X
Half sperm = Y
Haploid cell
Morphology of sperm
Head -contains nucleus & acrosome
Tail and Midpiece- contain mechanisms and enzymatic materials for motility
- Contains mitochondria
- Each sperm mitochondrion carries multiple copies of paternal mitochondria genome
- energy for sperm flagellar motility
- joins head and tail
Midpiece (neck)
Mobility or motility of spermatozoa
Tail
NFSTC
National forensic science technology center
Protamines
Arginine rich nuclear protein that replaces histones late in haploid phase of soermatogenesis
Two types of protamines are______
P1 & P2
Aspermia
No ejaculation (absence of spermatozoa)
- infertility
- drug use
- prostate surgery
Azoospermia
Lack of sperm cells in the semen (no spermatozoa)
- temporary or permanent
- vasectomy
- drugs
- alcoholism
- high fever
Oligospermia (asthenozoospermia)
Fewer number of spermatozoa
Teratozoospermia
Increased abnormal forms of sperm
Oligoasthenoteratozoospermia
Spermatozoa variables all subnormal (OAT)
Leucocytospermia
Increased leukocytes in semen
Necrozoospermia
All sperm non viable or non motile
Globozoospermia
Sperm heads lack acrosome caps and can’t fertilize
rare*
Phagocytic
Eats other cells
Protease
Break up of proteins
high concentration in semen and liquefies seminal fluid
- serine protease
- produced by epithelial cells & found in seminal fluid
PSA
Protease
Break up of proteins
high concentration in semen and liquefies seminal fluid
- serine protease
- produced by epithelial cells & found in seminal fluid
PSA
Protease
Break up of proteins
high concentration in semen and liquefies seminal fluid
- serine protease
- produced by epithelial cells & found in seminal fluid
PSA
Y shaped proteins
Immunoglobulin G (IgG)
ELISA
Enzyme linked immunosorbent assay
RSID
Rapid Stain Identification Semen Test
ABAcard P30 detects_____
PSA
RSID detects ______
Semenogelin (Sg)
3 major gel-forming proteins
Fibronectin
Semenogelin I
Semenogelin II
Semenogelin I
Disulfide linked complex of ~52-kDa protein
Semenogelin II
Two forms of an Sg I related protein of ~71 and ~76 kDa
Seminiferous tubules
Where sperm is being produced
Epididymis
A coiled tube next to each testis and receives sperm from the seminiferous tubules
Three phases of spermatogenesis
Spermatocytogenesis
Diploid cell meiosis
Spermiogenesis
Spermatocytogensis
Immature spermatogonia undergo mitotic division
*where mitosis takes place
Diploid cells -Meiosis
Diploid number present in first phase becomes haploid, 23 chromosomes
*secondary spermatocytes (haploid spermatids)
Spermiogenesis
Spermatids undergo morphological changes
*final phase-spermatids are haploid, see acrosome form & elongated
Spermiogenesis
Midpiece - mitochondria
Acrosome - enzymatic knife used to penetrate the oocyte
Nucleus - haploid chromosomes
Vas deferens
- Main vesicle for carrying sperm
- travels out scrotum–> into abdomen–> & passes behind urinary bladder–> expands to form expanded end part (ampulla)
Ampulla =bulb shape
Urethra
Carries semen (sperm) through penis during intercourse and during urination passes through it
*two different functions, do not occur at the same time
SRY
Sex determining region
*Y is smaller then X
ZFX
Zinc fingerprint protein
- located on X chromosome
- extremely important in humans
Danes method
Keratin
Prekeratin
Mucin
Ayoub-Shklar
Differentiate skin epithelial cells from buccal and vaginal
VAP
Vaginal acid phosphatase
SAP
Seminal acid phosphatase
ELISA
Enzyme linked immunosorbent assay
KPIC
Kernechtrot Picroindigocarmine stain
3 methods Fertility and Sterility
Shorr
Papanicolau (papsmear)
Testsimplets