DNA part 2

Question 1

Inhibitor for blood

Answer 1

Heme

Question 2

Inhibitors can inhibit the enzyme _____

Answer 2

Polymerase, Mg, or DNA

Question 3

Human nuclear DNA is not found in which cell type?

Answer 3

Red blood cells

Question 4

What type of strand is PCR?

Answer 4

Double stranded or single stranded

Question 5

What type of strand is Chelex?

Answer 5

Single stranded

Question 6

Reagents in Differential Extraction

Answer 6

Lysis Buffer
SDS
Pro K
DTT
EDTA
Buffer/Ions

Question 7

Chemicals and proteins added to the sample that facilitate lysis of the cell membrane and effective removal of impurities

Answer 7

Lysis Buffer

Question 8

SDS

Answer 8

Sodium dodecyl sulfate (detergent)

Question 9

Pro K

Answer 9

Proteinase K (digest proteins and chops it up)

Question 10

DTT

Answer 10

dithiothreitol

(Differential extraction, reads the S-S bond and allows the protein to break apart) -sperm heads broken down

reducing agent

Question 11

EDTA

Answer 11

Ethylenediaminetetraaceticacid (chelates metal ions that can degrade DNA and cause hydrolysis of DNA molecule)

Question 12

Buffer/Ions

Answer 12

Examples Tris-HCl and NaCl (maintains pH; ions helps later in the downstream)

Question 13

Two basic steps for isolation of DNA

Answer 13

~breaking open of the organism or cell that contains DNA

~separation of the DNA from other cellular components

~remove inhibitors

Question 14

Extraction techniques

Answer 14

Chelex
FTA
Robotic
Organic

Question 15

What type of strand is RFLP?

Answer 15

Single stranded

Question 16

PCI/PCIA

Answer 16

Phenol Chloroform Isoamyl Alcohol

gets rid of proteins