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RECALLS - MEDTECH 2021 > SEROLOGY > Flashcards

SEROLOGY Flashcards

1
Q

ANTIGEN EXCESS

A

Postzone phenomenon

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2
Q

ANTIBODY EXCESS

A

Prozone phenomenon

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3
Q

zone of equivalnce

A

maximum precipitation occurs

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4
Q

ideal pH for agglutination reaction

A

6.5 to 7.5

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5
Q

uses BACTERIA as INERT PARTICLES to which antibodies are attached

A

Coagglutination

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6
Q

ANTIBODY is attached to carrier particle

A

Reverse passive agglutination

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7
Q

ANTIGEN is attached to carrier particle

A

Passive Agglutination

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8
Q

antigens are naturally found on red cell

A

Hemagglutination

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9
Q

antigens are naturally found on the surface of the particle

A

Direct Agglutination

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10
Q

Lack of agglutination is an indicator of positive reaction

A

Agglutination inhibition

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11
Q

detects non-agglutinating antibody by means of coupling with a second antibody

A

Antiglobulin-Mediated Agglutination

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12
Q

In red cell Ag-Ab reactions small agglutinates, turbid background (25% agglutination) is graded as

A

1+

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13
Q

In red cell Ag-Ab reactions large agglutinate, clear background (75%a gglutination)

A

3+

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14
Q

In red cell Ag-Ab reactions a weakly positive is characterized by

A

tiny agglutinates with turbid background

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15
Q

similar to immunoelectrophoresis except that in this test , the ANTISERUM is LAYERED ON THE MEDIUM

A

Immunofixation Electrophoresis

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16
Q

Laurell Rocket Immunoelectrophoresis

A

Radial immunodiffusion + electrophoresis = rocket band is formed

17
Q

Height/apex of the rocket is ___________ to the antigen concentration

A

DIRECTLY PROPORTIONAL

18
Q

A precipitation reaction test for detection of BENCE-JONES PROTEINS

A

Immunoelectrophoresis

19
Q

serum electrophoresed to separate protein fractions; antiserum is added to the trough; change in shape of PRECIPITIN ARCS

A

Immunoelectrophoresis

20
Q

Both antigen and antibody diffuse independently through a semisolid medium in TWO DIMENSIONS

A

Ouchterlony Double Diffusion

21
Q

Antibody is incorporated into the gel medium; antigen is applied on the medium, diffuse ad reacts with the antibody, producing the PRECIPITIN BAND

A

Oudin Single Diffusion

22
Q

d=log Antigen concentration

A

FAHEY MCKELVEY/KINETIC METHOD RID

23
Q

FAHEY MCKELVEY/KINETIC METHOD RID

A

measurement is TAKEN BEFORE THE POINT OF EQUIVALENCE

24
Q

d^2= Antigen concentration

A

MANCINI/ENDPOINT METHOD RID

25
Q

MANCINI/ENDPOINT METHOD RID

A

antigen is allowed to diffuse to COMPLETION

26
Q

Diffusion time of IgG in MANCINI mtd

A

24 hours

27
Q

Diffusion time of IgM in MANCINI mtd

A

50-72 hours

28
Q

measured in Radial Immunodiffusion

A

diameter of precipitation is measured

29
Q

type of immunodiffusion in Radial Immunodiffusion

A

Single diffusion in 2-dimension

30
Q

type of immunodiffusion in Ouchterlony method

A

Double diffusion in 2 dimensions

RECALLS - MEDTECH 2021 (11 decks)

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