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RECALLS - MEDTECH 2021 > Microbiology > Flashcards

Microbiology Flashcards

1
Q

Buffered Charcoal yeast Extract (BCYE) agar

A

Legionella spp

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2
Q

Ashdown medium

A

Burkholderia pseudomallei

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3
Q

Alkaline peptone broth

A

Vibrio

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4
Q

Bordet Gengou (potato blood glycerol agar)

A

Bordetella pertussis

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5
Q

Cefsulodin-irgasan-novobiocin (CIN)

A

Yersinia

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6
Q

flat wrinkled purple colonies in ashdown medium

A

burkholderia pseudomallei

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7
Q

Cetrimide agar

A

Pseudomonas aeruginosa

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8
Q

Cystine blood glucose agar

A

Francisella tularensis

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9
Q

Czapek’s agar

A

Aspergillus

Nocardia asteroides

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10
Q

Loeffler’s serum medium

A

C. diptheriae

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11
Q

SMAC (MaConkey Sorbitol) agar

A

E. coli O157:H7

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12
Q

Mannitol Salt agar

A

S. aureus

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13
Q

Mueller Hinton Agar is used in

A

Susceptibility testing

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14
Q

Skirrow agar

A

Campylobacter spp

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15
Q

Tindsdale agar

A

C. diptheriae

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16
Q

Selenite broth

A

Salmonella spp

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17
Q

Regan-Lowe agar

A

Bordetella pertussis

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18
Q

Petragnani medium

A

Mycobacteria

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19
Q

PEA

A

Gram positive cocci & anaerobic gram negative bacilli

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20
Q

XLD

A

Salmonella-Shigella vs other enteric

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21
Q

agar made of extracts of meat& soybeans

A

nutrient agar

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22
Q

agar that inhibit growth of other bacteria

A

Selective

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23
Q

agar with added factors like blood, vitamins and yeast extracts

A

Enriched

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24
Q

obligate aerobes grow on ____ of broth

A

top of broth

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25
Q

top of broth contains ___O2

A

20-21%

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26
Q

Microaerophiles require ___ oxygen

A

5%

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27
Q

blood agar is composed of

A

nutrient agar + 5% sheep’s blood

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28
Q

clear medium for mycobacteria

A

middlebrook 7H10/7H11

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29
Q

Suitable transport medium for bacteria & viruses

A

Stuart’s medium

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30
Q

purpose of reducing agent in medium

A

prevent oxidation

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31
Q

Tellurite function

A

Inhibitor

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32
Q

salmonella-shigella agar inhibitors

A

Bile salts, brilliant green

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33
Q

indicator in XLD agar

A

phenol red

34
Q

inoculation used in isolation of desirable bacteria

A

streaking

35
Q

of quadrants on a culture medium streaked for stool culture

A

4

36
Q

0.5 McFarland standard solution

A

1.175% BaCl2 + 1% H2SO4

37
Q

To isolate an organism from a liquid specimen

A

Pour plate

38
Q

types of growth on agar slants

A 
filiform
echinulate
beaded
effuse
arborescent 
rhizoid
39
Q

most common method for AST determination

A

Kirby Bauer method

40
Q

lowest concentration of antibiotic that inhibits microbial growth

A

Minimum inhibitory concentration

41
Q

lowest concentration of antibiotic that kills 99.9% of bacteria

A

Minimum bactericidal concentration

42
Q

zone diameter of inhibition is _____ proportional to MIC

A

INVERSELY

43
Q

has series of antibiotic concentrations for determining antimicrobial susc. in a broth

A

broth dilution

44
Q

incubator is set at what temp for most human pathogens

A

35’C

45
Q

Phase in which bacterial cells INCREASE IN SIZE not in number

A

Lag phase

46
Q

capnophilic organism require ___% CO2

A

5-10%

47
Q

Most common failure concerning GASPAK

A

Inactivated catalyst

48
Q

differentiation of GRAM STAINING occurs in

A

DECOLORIZATION

49
Q

for better visualization of LEGIONELLA SPP., Safranin is replaced with ___

A

0.1% fuschin

50
Q

stain retained by acid fast organisms

A

Carbolfuchsin

51
Q

color of AFB

A

pink/red

52
Q

color of non-AFB

A

blue

53
Q

stain for Endospores

A

Dorner
Wirtz
Conklin
Schaeffer-fulton***

54
Q

Acridine orange***

Feulgen

A

stains for nucleic acid

55
Q

Leifson***
Gray
Fisher
Conn

A

flagellar stain

56
Q

Polar bodies stain

A

Wayson

57
Q

stain for Rickettsia

A

Gimenez stain***

Macchiavello stain

58
Q

Spirochete stain

A

Dieterle silver stain***

59
Q

Mycobacterium leprae stain

A

Fite-faraco

60
Q

fite faraco counterstain

A

hematoxylin

61
Q

capsular stains

A

Hiss
Anthony
Welch
Gin

62
Q

Dyar stain

A

cell wall

63
Q

negri bodies stain

A

Seller’s stain

64
Q

Mycoplasma colony stain

A

Diene’s stain

65
Q

ascaris egg stain

A

malachite green

66
Q

filters exhausted air only

A

BSC Class I

67
Q

BSC Class II

A

filters exhausted air and recirculated air

68
Q

70% recirculated through HEPA

A

BSC Class IIA

69
Q

biosafety level for Marburg and congo-crimean hemorrahigc fever

A

Biosafety level 4

70
Q

biosafety level for HBV, HIV, Salmonella, Toxoplasma

A

Biosafety level 2

71
Q

protection from droplet nuclei

A

Mask

72
Q

bacteria is attracted to _____ environment

A

moist

73
Q

five most frequently acquired laboratory infections

A
  1. Shigellosis
  2. Salmonellosis
  3. Tuberculosis
  4. Brucellosis
  5. Hepatitis
74
Q

fumigant used for cold sterilization of surgical instrumentation

A

Ethylene oxide

75
Q

disinfection

A

killing PATHOGENIC microorganism

76
Q

Sterilization

A

killing of all microorganism

77
Q

Certified Milk contains _____ bacteria

A

10,000 or less bacteria per mL

78
Q

boiling is

A

non-sporicidal

79
Q

concentration of H2O2 for cleansing wounds

A

3%

80
Q

throat swab for anaerobic culture is sent to the lab. what should the medtech on duty do?

A

Reject

RECALLS - MEDTECH 2021 (11 decks)

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