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RECALLS - MEDTECH 2021 > Microbiology > Flashcards

Microbiology Flashcards

1
Q

Buffered Charcoal yeast Extract (BCYE) agar

A

Legionella spp

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2
Q

Ashdown medium

A

Burkholderia pseudomallei

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3
Q

Alkaline peptone broth

A

Vibrio

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4
Q

Bordet Gengou (potato blood glycerol agar)

A

Bordetella pertussis

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5
Q

Cefsulodin-irgasan-novobiocin (CIN)

A

Yersinia

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6
Q

flat wrinkled purple colonies in ashdown medium

A

burkholderia pseudomallei

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7
Q

Cetrimide agar

A

Pseudomonas aeruginosa

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8
Q

Cystine blood glucose agar

A

Francisella tularensis

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9
Q

Czapek’s agar

A

Aspergillus

Nocardia asteroides

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10
Q

Loeffler’s serum medium

A

C. diptheriae

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11
Q

SMAC (MaConkey Sorbitol) agar

A

E. coli O157:H7

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12
Q

Mannitol Salt agar

A

S. aureus

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13
Q

Mueller Hinton Agar is used in

A

Susceptibility testing

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14
Q

Skirrow agar

A

Campylobacter spp

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15
Q

Tindsdale agar

A

C. diptheriae

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16
Q

Selenite broth

A

Salmonella spp

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17
Q

Regan-Lowe agar

A

Bordetella pertussis

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18
Q

Petragnani medium

A

Mycobacteria

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19
Q

PEA

A

Gram positive cocci & anaerobic gram negative bacilli

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20
Q

XLD

A

Salmonella-Shigella vs other enteric

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21
Q

agar made of extracts of meat& soybeans

A

nutrient agar

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22
Q

agar that inhibit growth of other bacteria

A

Selective

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23
Q

agar with added factors like blood, vitamins and yeast extracts

A

Enriched

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24
Q

obligate aerobes grow on ____ of broth

A

top of broth

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25
top of broth contains ___O2
20-21%
26
Microaerophiles require ___ oxygen
5%
27
blood agar is composed of
nutrient agar + 5% sheep's blood
28
clear medium for mycobacteria
middlebrook 7H10/7H11
29
Suitable transport medium for bacteria & viruses
Stuart's medium
30
purpose of reducing agent in medium
prevent oxidation
31
Tellurite function
Inhibitor
32
salmonella-shigella agar inhibitors
Bile salts, brilliant green
33
indicator in XLD agar
phenol red
34
inoculation used in isolation of desirable bacteria
streaking
35
of quadrants on a culture medium streaked for stool culture
4
36
0.5 McFarland standard solution
1.175% BaCl2 + 1% H2SO4
37
To isolate an organism from a liquid specimen
Pour plate
38
types of growth on agar slants
``` filiform echinulate beaded effuse arborescent rhizoid ```
39
most common method for AST determination
Kirby Bauer method
40
lowest concentration of antibiotic that inhibits microbial growth
Minimum inhibitory concentration
41
lowest concentration of antibiotic that kills 99.9% of bacteria
Minimum bactericidal concentration
42
zone diameter of inhibition is _____ proportional to MIC
INVERSELY
43
has series of antibiotic concentrations for determining antimicrobial susc. in a broth
broth dilution
44
incubator is set at what temp for most human pathogens
35'C
45
Phase in which bacterial cells INCREASE IN SIZE not in number
Lag phase
46
capnophilic organism require ___% CO2
5-10%
47
Most common failure concerning GASPAK
Inactivated catalyst
48
differentiation of GRAM STAINING occurs in
DECOLORIZATION
49
for better visualization of LEGIONELLA SPP., Safranin is replaced with ___
0.1% fuschin
50
stain retained by acid fast organisms
Carbolfuchsin
51
color of AFB
pink/red
52
color of non-AFB
blue
53
stain for Endospores
Dorner Wirtz Conklin Schaeffer-fulton***
54
Acridine orange*** | Feulgen
stains for nucleic acid
55
Leifson*** Gray Fisher Conn
flagellar stain
56
Polar bodies stain
Wayson
57
stain for Rickettsia
Gimenez stain*** | Macchiavello stain
58
Spirochete stain
Dieterle silver stain***
59
Mycobacterium leprae stain
Fite-faraco
60
fite faraco counterstain
hematoxylin
61
capsular stains
Hiss Anthony Welch Gin
62
Dyar stain
cell wall
63
negri bodies stain
Seller's stain
64
Mycoplasma colony stain
Diene's stain
65
ascaris egg stain
malachite green
66
filters exhausted air only
BSC Class I
67
BSC Class II
filters exhausted air and recirculated air
68
70% recirculated through HEPA
BSC Class IIA
69
biosafety level for Marburg and congo-crimean hemorrahigc fever
Biosafety level 4
70
biosafety level for HBV, HIV, Salmonella, Toxoplasma
Biosafety level 2
71
protection from droplet nuclei
Mask
72
bacteria is attracted to _____ environment
moist
73
five most frequently acquired laboratory infections
1. Shigellosis 2. Salmonellosis 3. Tuberculosis 4. Brucellosis 5. Hepatitis
74
fumigant used for cold sterilization of surgical instrumentation
Ethylene oxide
75
disinfection
killing PATHOGENIC microorganism
76
Sterilization
killing of all microorganism
77
Certified Milk contains _____ bacteria
10,000 or less bacteria per mL
78
boiling is
non-sporicidal
79
concentration of H2O2 for cleansing wounds
3%
80
throat swab for anaerobic culture is sent to the lab. what should the medtech on duty do?
Reject

RECALLS - MEDTECH 2021 (11 decks)

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