SEROLOGICAL TEST

Question 1

Provides enough information to name the species of the fungal organism

Answer 1

SEROLOGICAL TEST

Question 2

key elements that needed to improve management of invasive fungal infections

Answer 2

Rapid diagnosis
Early initiation of antibiotic fungal therapy

Question 3

• Time consuming; mold cultures grow after several weeks.
• It has low sensitivity due to the low concentration of the organism in the samples infested.

Answer 3

Culture methods

Question 4

• Detection of specific host immune responses to fungal antigens using immunological reagents
• Detection and quantitation of specific fungal metabolite byproducts

Answer 4

Non-culture methods

Question 5

Targets for serological tests:

Answer 5

● Antigen
● Antibody
● Metabolites

Question 6

Types of serological tests

Answer 6

Agglutination
Immunodiffusion
Complement Fixation Test
Enzyme Linked Immunosorbent Assay
Lateral Flow Assay

Question 6

Good for Cryptococcosis
Detect polysaccharide antigen
Qualitative & semi-quantitative

Answer 6

LATEX AGGLUTINATION

Question 7

Detection of Heat labile glycoprotein, Mannan antigen and anti–Mannan antibody

Answer 7

Invasive Candidiasis

Question 8

is a component of cell wall

Answer 8

mannan

Question 9

latex agglutination should be treated with

Answer 9

Sodium metaperiodate

Question 9

latex agglutination should be pretreating serum with

Answer 9

2 beta mercaptoethanol

Question 10

Look for antibodies of specific antigens

Answer 10

IMMUNODIFFUSION

Question 11

Active infection

Answer 11

(+) M and H

Question 11

Early/Chronic Infection

Answer 11

(+) M only

Question 11

COMPLEMENT FIXATION TEST: Detection of IgE antibodies

Answer 11

Aspergillosis

Question 11

COMPLEMENT FIXATION TEST:
◆ Ab detected against M & H Ags
◆ high sensitivity, low specificity

Answer 11

Histoplasmosis

Question 12

ELISA: Qualitative or Screening assay

Answer 12

Cryptococcosis/ Blastomycosis/ Histoplasmosis/Coccidioidomycosis

Question 12

COMPLEMENT FIXATION TEST: Detects IgG Ab to Coccidioides culture filtrate

Answer 12

Coccidioidomycosis

Question 12

Presumptive diagnosis of Invasive candidiasis by Mannan Ag & Anti-Mannan Ab Assay

Answer 12

Candidiasis

Question 12

ELISA: Allergic = detection of IgE Ab
◆ Invasive = Screening test; detects
galactomannan

Answer 12

Aspergillosis

Question 13

➔ Adjunct in diagnosis of invasive fungal infections
➔ Pan fungal marker

Answer 13

1,3 β D-Glucan Assay

Question 13

Detection of metabolites

Answer 13

D-Arabinitol
Secreted Aspartyl Proteinase

Question 13

Rapidly eliminated by immune complexes & endocytosis by Kuppfer cells

Answer 13

Mannan

Question 13

makes use of several different target genes to identify an organism

Answer 13

Multiplex PCR

Question 13

Screening test & determination of endpoint titres

Answer 13

Cryptococcosis

Question 13

component in majority of fungal cell walls

Answer 13

Exo antigen

Question 14

makes use of two sets of primers

Answer 14

Nested PCR

Question 15

○ Widely used for Candida albicans
○ Microbroth dilution MIC test
○ Tests several species of Candida against different antifungal agents
○ Reliable sensitivity test

Answer 15

Sensititre Yeast One

Question 15

Allows the physician to choose the right antifungal agent not really required

Answer 15

E-TEST Agar MIC Method

Question 15

○ Microbroth dilution breakpoint test
○ Allows us to identify which concentration of
drugs is susceptible

Answer 15

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