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MYCOLOGY AND VIROLOGY > L2: page 11 > Flashcards

L2: page 11 Flashcards

1
Q

COMPLEMENT FIXATION TEST: Detection of IgE antibodies

A

Aspergillosis

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2
Q

COMPLEMENT FIXATION TEST:
Ab detected against M & H Ags
high sensitivity, low specificity

A

Histoplasmosis

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3
Q

ENZYME LINKED IMMUNOSORBENT ASSAY: Qualitative or Screening assay

A

Cryptococcosis/ Blastomycosis/
Histoplasmosis/Coccidioidomycosis

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3
Q

COMPLEMENT FIXATION TEST: Detects IgG Ab to Coccidioides culture filtrate

A

Coccidioidomycosis

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4
Q

ENZYME LINKED IMMUNOSORBENT ASSAY: Aspergillosis - Screening test; detects galactomannan

A

Invasive

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4
Q

ENZYME LINKED IMMUNOSORBENT ASSAY: Aspergillosis - detection of IgE Ab

A

Allergic

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4
Q

Presumptive diagnosis of Invasive candidiasis by Mannan Ag & Anti-Mannan Ab Assay

A

Candidiasis

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4
Q

Detection of metabolites

A

D-Arabinitol
Secreted Aspartyl Proteinase

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4
Q

LATERAL FLOW ASSAY:
Aspergillosis - detects GM

A

invasive

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4
Q

rapidly eliminated by immune complexes & endocytosis by Kuppfer cells

A

Mannan

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4
Q

LATERAL FLOW ASSAY:
Screening test & determination of endpoint titres
Caused by: C. neoformans & C. gattii

A

Cryptococcosis

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4
Q

LATERAL FLOW ASSAY:
Aspergillosis - detects IgE Ab

A

allergic

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4
Q

➔ Adjunct in diagnosis of invasive fungal infections
➔ Pan fungal marker

A

1,3 β D-Glucan Assay

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4
Q

component in majority of fungal cell walls

A

Exo antigen

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4
Q

makes use of several different target genes to identify an organism

A

multiplex polymerase chain reaction

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4
Q

makes use of two sets of primers

A

nested polymerase chain reaction

4
Q

Molecular Amplifications; most fungal elements
amplified using target genes are:

A

candida
pneumocystis
aspergillus

4
Q

○ Allows the physician to choose the right antifungal agent
○ not really required

A

E-TEST Agar MIC Method

4
Q

Widely used for Candida albicans
○ Microbroth dilution MIC test
○ Tests several species of Candida against
different antifungal agents
○ Reliable sensitivity test

A

Sensititre Yeast One

MYCOLOGY AND VIROLOGY (4 decks)

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