L2: page 11 Flashcards
COMPLEMENT FIXATION TEST: Detection of IgE antibodies
Aspergillosis
COMPLEMENT FIXATION TEST:
Ab detected against M & H Ags
high sensitivity, low specificity
Histoplasmosis
ENZYME LINKED IMMUNOSORBENT ASSAY: Qualitative or Screening assay
Cryptococcosis/ Blastomycosis/
Histoplasmosis/Coccidioidomycosis
COMPLEMENT FIXATION TEST: Detects IgG Ab to Coccidioides culture filtrate
Coccidioidomycosis
ENZYME LINKED IMMUNOSORBENT ASSAY: Aspergillosis - Screening test; detects galactomannan
Invasive
ENZYME LINKED IMMUNOSORBENT ASSAY: Aspergillosis - detection of IgE Ab
Allergic
Presumptive diagnosis of Invasive candidiasis by Mannan Ag & Anti-Mannan Ab Assay
Candidiasis
Detection of metabolites
D-Arabinitol
Secreted Aspartyl Proteinase
LATERAL FLOW ASSAY:
Aspergillosis - detects GM
invasive
rapidly eliminated by immune complexes & endocytosis by Kuppfer cells
Mannan
LATERAL FLOW ASSAY:
Screening test & determination of endpoint titres
Caused by: C. neoformans & C. gattii
Cryptococcosis
LATERAL FLOW ASSAY:
Aspergillosis - detects IgE Ab
allergic
➔ Adjunct in diagnosis of invasive fungal infections
➔ Pan fungal marker
1,3 β D-Glucan Assay
component in majority of fungal cell walls
Exo antigen
makes use of several different target genes to identify an organism
multiplex polymerase chain reaction
makes use of two sets of primers
nested polymerase chain reaction
Molecular Amplifications; most fungal elements
amplified using target genes are:
candida
pneumocystis
aspergillus
○ Allows the physician to choose the right antifungal agent
○ not really required
E-TEST Agar MIC Method
Widely used for Candida albicans
○ Microbroth dilution MIC test
○ Tests several species of Candida against
different antifungal agents
○ Reliable sensitivity test
Sensititre Yeast One
