serological and molecular DIAGNOSIS OF SOME PARASITIC AND FUNGALl infetions Flashcards
The detection of galactomannan and B, D- glucan is for
a. Aspergilossis
b. cryptococcus
c. candidiasis
d. histoplasmosis
aspergilossis
- The detection of galactomannan in serum by EIA has increased the ability to diagnose invasive aspergillosis.
- Another assay to detect invasive fungal infections, including aspergillosis, is measuring β-D-glucan (BDG) in serum. BDG is a component of the cell wall of most fungi
test can be used as both a qualitative screening test and a semiquantitative test to detect antibodies to C. neoformans.
a. cyrptococcus latex agglutination test
b. tube aglutination test
c. india ink
d. indirect immunofluorescent assay cryptococcus
Tube agglutination test for Cryptococcus neoformans
- qualitative screening test and a semiquantitative test to detect antibodies to C. neoformans.
A serological test that detects the polysaccharide capsule antigen in serum and CSF of patients with suspected infection with Cryptococcus neoformans
.a. cyrptococcus latex agglutination test
b. tube aglutination test
c. india ink
d. indirect immunofluorescent assay cryptococcus
india ink
- test for suspected infection of C. neoformans
- create a halo around the yeast cell
what fungal infection used for mannan and anti mannan antibodies for detection
a. Aspergilossis
b. cryptococcus
c. candidiasis
d. histoplasmosis
aspergilosis
- detection of galactomannan and B, D- glucan
Candidiasis
- MANNAN AND ANTI MANNAN
what test currently used for detection of candida albicans from other candida albicans
a. india ink
b. tube agglutination assay
c. peptide nuclear acid fluorescent in situ hybridization
Peptide nuclear acid fluorescent in situ hybridization (PNA-FISH)
- This technique utilizes small PNA polymer probes that are labeled with a fluorescent dye to identify PNA sequences on chromosomes. T
- This procedure is currently used to differentiate Candida albicans from other Candida species.
Cross-reactions in patients with acute histoplasmosis will occur as false-positive reactions
a. Cryptococcal latex agglutination tests
b. Complement fixation tests for Coccidiodomycosis
c. tube agglutination tests
d. tube precipitation tests
Complement fixation tests for Coccidiodomycosis (IgG tests)
- acute histoplasmosis
Cryptococcal Latex agglutination test
- Cross-reactions may occasionally occur due to rheumatoid factor (RF)
precipitating IgM antibodies and it is also a test for early indication of primary infection of coccidiodomycosis
a. Cryptococcal latex agglutination tests
b. Complement fixation tests for Coccidiodomycosis
c. tube agglutination tests
d. tube precipitation tests
Tube Precipitation Test for Coccidiodomycosis (IgM test)
- Precipitating IgM antibodies appear in 1 to 3 weeks
- a positive TP test can be an early indication of a primary infection.
gold standard test for Toxoplasmosis
a. Sabin feldman dye test
b. ELISA
c. EIA
d. PCR
Sabin-Feldman Dye test
- Considered as the gold standard test
- Can be used to detect IgG antibodies
- Principle: NEUTRALIZATION
- indicator : methylene blue
(-) no color
(+) blue color
widely used immunoasay for Toxoplasmosis
a. ELISA
b. EIA
c. Chemiluminescence
d. IFA
IFA
- widely used
EIA
- method of choice
- more sensitive
- less difficult to perform
- easierto interpret
what test is for t. gondii DNA in CSF
a. PCR
b. EIA
c. Chemiluminescence
d. IFA
Polymerase chain reaction (PCR)
- used to detect the presence or absence of T. gondii DNA in fresh or frozen biopsy tissue, CSF, amniotic fluid, serum, or plasma
- PCR is the method of choice to detect T. gondii DNA in CSF
For detection of toxoplasma antibodies IN SERUM, the most commonly used serological method is
a. PCR
b. EIA
c. Chemiluminescence
d. IFA
eia
- for serum
pcr
- csf
