Labeled immunoassays Flashcards
unlabeled analytes that are made up in known concentrations of the substance to be measured
a. standard
b. calibrators
c. competitive immunoassays
d. aota
standard is also known as calibrators
- unlabeled analytes that are made up in known concentrations of the substance to be measured
all reactants are mixed together simultaneously
a. competitive immunoassays
b. non competitive immunoassays
c. heterogenous immunoassays
d. homogenous immunoassays
competitive immunoassay
- all reactants are mixed together simultaneously
- Labeled antigen COMPARES with UNlabeled patient antigen
noncompetitive immunoassay
- capture antibody
an immunoassay that has no separation step
a. competitive immunoassays
b. non competitive immunoassays
c. heterogenous immunoassays
d. homogenous immunoassays
homogenous immunoassays
- No separation step
- principle : Decrease enzyme activity
- less sensitive than hetero
an immunoassay requires washing
a. competitive immunoassays
b. non competitive immunoassays
c. heterogenous immunoassays
d. homogenous immunoassays
heterogenous
- enzyme is used as a label
- requires a Separation step such as Washing
who developed RIA
a. ellie metchinikoff
b. rosalyn yallow
c. kuhler
d. koch
rosalyn yallow
what instrument used to measure RIA
a. spectrophotometer
b. scintillation counter
c. nota
Radioactivity is measured by a SCINTILLATION COUNTER (GAMMA)
amount of bound labeled antibody in Non competitive RIA is _________ proportional to the amount of patient analyte
a. inversely
b. direct
non competitive RIA
amount of bound labeled antibody is DIRECTLY proportional to the amount of patient analyte
COMPETITIVE RIA
the amount of label in the bound phase is INVERSELY PROPORTIONAL to the amount of patient antigen present
what are the common enzymes used in EIA
a. hpo
b. G6PD
c. HRP
d. lysozyme
ENZYME IMMUNOASSAY (EIA) - USES ENZYMES AS LABELS FOR IMMUNOASSAYS SUCH AS HRP/ HPO (MOST COMMONLY USED) , GLUCOSE OXIDASE , ALP, G6OD , B-GALACTOSIDASE
answer: hrp/ hpo
if antigen is bound to a solid phase, these assay is called
a. sandwich immunoassay
b. standard immunoassay
if antigen is bound to a solid phase, these assay is called STANDARD / INDIRECT ELISA
if antibody is bound to a solid phase, these assay is called SANDWICH / CAPTURE ASSAYS
enzymes is measured by
a. spectrophotometer
b. scintillation counter
c. nota
Radioactivity is measured by a SCINTILLATION COUNTER (GAMMA)
Enzyme immunoassay (EIA) - is measured by SPECTROPHOTOMETER
which of the following test belongs to homogenous EIA
a. EMIT
B. RAST
C. IRMA
D. RIA
NON COMPETITIVE RIA
- EXAMPLE: IRMA (Immunoradiometric assay)
RAST ( Radio allegro sorbent test)
example of a HOMOGENOUS IMMUNOASSAY
- Enzyme - multiplied immunoassay technique ( EMIT)
this immunoassay principle is based on change in enzyme activity as specific antigen - antibody combination occurs
a.homogenous EIA
b, competitive EIA
c.direct immunofluorescent assay
homogenous EIA
- principle is based on change in enzyme activity as specific antigen - antibody combination occurs
competitive EIA
- based on the principle of RIA
two enzyme frequently used in homogenous EIA
a. hrp
b. alp
c. malate dehydrogenase
d. G6pd
two enzyme frequently used in homogenous EIA
- malate dehydrogenase
- g6pd
which of the following fluorochromes are commonly used
a. phycobiliprotein
b. europium
c. fitc
d, tritc
two most commonly used fluorochromes
A. Fluorescein isothiocyante (FITC)
- emits apple green at 520 nm
B. tetramethyl rhodamine isothiocyanate (TRITC)
- emits red color at 585 nm
other fluorochromes are Phylocobiliprotein, europium (b- naphthyl trifluoroacteone) and lucifer yellow VS
antibody that is conjugated with fluorescent tag is added directly to unknown antigen fixed to a microscope slide
a. DFA
b. DAT
c. IFA
d. FPIA
direct immunofluorescent assay (DFA)
- antibody that is conjugated with fluorescent tag is added directly to UNKNOWN antigen fixed to a microscope slide
