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serology > serological and molecular detection of bacterial infetions > Flashcards

serological and molecular detection of bacterial infetions Flashcards

1
Q

what is test for detection of streptococcal antigen

a. streptozyme kit testing
b. ASO testing
c. anti- dnase testing
d. LFA

A

detection of streptococcal antigen
- LATERAL FLOW IMMUNOCHROMATOGRAPHIC ASSAY

LFA
- widely used in opd, physician offices and urgent care facilities for rapid diagnosis of streptococcal pharyngitis

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2
Q

what is the test for streptococcal is a slide agglutination screening test for detection of antibodies to several streptococcal antigen

a. streptozyme kit testing
b. ASO testing
c. anti- dnase testing
d. LFA

A

Streptozyme kit testing

  • slide agglutination screening test for detection of antibodies to several streptococcal antigen
  • sheep RBCs are coated with STREPTOLYSIN, STREPTOKINASE, HYALURONIDASE, DNASE AND NADASE
  • HEMAGGLUTINATION IS POSITIVE
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3
Q

IN ASO agglutination test what is the significant result

a. <200 IU/ml
b. 320 IU/ml
c. >200IU/ml
d. nota

A

IN ASO agglutination test / latex agglutination testing
>200 IU/ml significant result

traditional / classic aso titer tubemethod
- titer of 160 Tood units or below = significant
-moderately elevated if the titer is atleast 240 Todd in adults =and 320 in child
-

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4
Q

Test for H.pylori that determines the patient’s bacterial load

a. rt - pcr
b. urea breath tests
c/ serological test
d. CLOtest

A

molecular method - RT PCR TEST

  • It can detect the presence of the organism in FECAL SAMPLES , cannot distinguish between living and dead H pylori
  • It determines the patient’s bacterial load
  • has shown good correlation with the urea breath test
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5
Q

PRIMARY ANTIBODY THAT CAN BE FOUND IN H. PYLORI

A. IgM
B. IgG
C. IgA
D. IgE

A

Serological testing

  • is a primary screening method of determining infection with H pylori.
  • infections from this organism result in production of IgG, IgA, and IgM antibodies
  • IgG is the primary antibody found.
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6
Q

which of the following dermatological manifestation is associated with Mycoplasma pneumoniae

a. Stevens- johnson syndrome
b. erythema multiforme major
c. raynaud syndrome

A

M.PNEUMONIAE
It is associated with dermatological manifestation such as;

-   Stevens– Johnson syndrome, or erythema multiforme major
              ===  a condition in which the TOP OF THELAYER OF THE SKIN DIES AND SHEDS
  • Raynaud syndrome
    ==== a transient vasospasm of the digits in which the FINGERS TURN WHITE when exposed to the cold
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7
Q

The test is based on the on cross-reactivity of the patient’s antibodies with polysaccharide antigens present on OX19 and OX2 strain of Proteus vulgaris and the OX-K strain of Proteus mirabilis

A. INDIRECT IMMUNOFLUORESCENCE ASSAY
B. WEIL FELIX TEST
C. WESTERN BLOT TEST
D. ENZYME IMMUNOASSSAY

A

WEIL FELIX TEST

  • CROSS REACTIVITY
  • PRINCIPLE : DIRECT AGGLUTINATION
  • SIGNIFANT / POSITIVE TITER : 1:320

The gold standard for the serological diagnosis of RMSF is the indirect immunofluorescence assay (IFA

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8
Q

what is the test to confirm congenital syphillis

a, Wasserman test

b. western blot test
c. weil felix test
d. EIA

A

wasserman test
- 1st diagnostic blood test for syphillis

WESTERN BLOT TEST

  • recommended to confirm congenital syphilis
  • only for congenital syphillis

weil felix
- for ricketsiaa

EIA

  • useful in diagnosing congenital syphilis in infants because they look for the presence of IgM
  • monitoring therapy
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9
Q

Both a qualitative and quantitative slide flocculation test for serum and CSF

a. VDRL - Venereal Disease Research Laboratory Test
a. RPR- Rapid Plasma Reagin
b. TRUST- Toluidine Red Unheated Serum Test
c. USR- Unheated Serum Reagin
d. RST- Reagin Screen Test

A

VDRL

    • Both a qualitative and quantitative slide flocculation test for serum and CSF
  • Specimen: 50 ul or 0.05 mL serum HEATED at 56oC for 30 minutes to inactive complements
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10
Q

The cardiolipin-containing antigen suspension is bound to charcoal particles, which make the test easier to read.

a. VDRL - Venereal Disease Research Laboratory Test
B. RPR- Rapid Plasma Reagin
C. TRUST- Toluidine Red Unheated Serum Test
D USR- Unheated Serum Reagin
E. RST- Reagin Screen Test

A 
RPR
  -  contains CHARCOAL PARTICLES 
  - read macroscopically
  -  Specimen:     
               50 ul or 0.05ml serum that does not have to be heat-inactivated
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11
Q

Specific anti-treponemal antibodies in early or untreated early latent syphilis are predominantly _______ antibodies

a. IgM
b. IgG
c. IgD
d. Iga

A

Specific anti-treponemal antibodies in early or untreated early latent syphilis are predominantly immunoglobulin M (IgM) antibodies

The greatest elevation in IgG concentration is seen in secondary syphilis.

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12
Q

The greatest elevation in ____ concentration is seen in secondary syphilis.

a. IgM
b. IgG
c. IgD
d. Iga

A

Specific anti-treponemal antibodies in early or untreated early latent syphilis are predominantly immunoglobulin M (IgM) antibodies

The greatest elevation in IgG concentration is seen in secondary syphilis.

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13
Q

Involves mixing of patient serum with live, actively motile T.pallidum extracted from testicular chancre of a rabbit and complement

a. TPA
B. HEMAGGLUTINATION TESTS
C. FTA - ABS

A

TREPONEMA PALLIDUM IMMOBILIZATION TEST (TPI)

  • Involves mixing of patient serum with live, actively motile T.pallidum
  • • The STANDARD TEST to which other treponema test are evaluated
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14
Q

Formalinized, tanned sheep RBCs sensitized with antigen from Nichols strain

a. TPHA
B. TPPA
C. MHA- TP
D. HATTS

A

TPHA (T.pallidum hemagglutination assay)
- Tanned sheep RBCs coated with antigen from Nichols strain

TPPA (T.pallidum particle agglutination assay)
- GEL PARTICLESs sensitized with T.pallidum sonicate

MHA-TP (Micro hemagglutination assay for T.pallidum)

 - FORMLAIZED, tanned sheep RBCs SENSITIZED  with antigen from Nichols strain
 - it is performed using microtiter plates

HATTS (Hemagglutination Treponemal test for Syphilis)
- GLUTARALDEHYDE stabilized TURKEY RBC

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15
Q

Gel particles sensitized with T.pallidum sonicate

a. TPHA
B. TPPA
C. MHA- TP
D. HATTS

A

TPHA (T.pallidum hemagglutination assay)
- Tanned sheep RBCs coated with antigen from Nichols strain

TPPA (T.pallidum particle agglutination assay)
- GEL PARTICLESs sensitized with T.pallidum sonicate

MHA-TP (Micro hemagglutination assay for T.pallidum)

 - FORMLAIZED, tanned sheep RBCs SENSITIZED  with antigen from Nichols strain
 - it is performed using microtiter plates

HATTS (Hemagglutination Treponemal test for Syphilis)
- GLUTARALDEHYDE stabilized TURKEY RBC

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serology (6 decks)

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