SEMR only: QC and QA Flashcards
Pre-analytical phase
* Test requisition
* Processing of test requisition
* Collection of sample
* Sample transport
* Initial processing
* Transport to microbiology section
QUALITY ASSURANCE
Analytical phase
* Examination and workup of culture
* Interpretation of results
QUALITY ASSURANCE
Post- analytical phase
* Preparation and production of written report
* Distribution of results to the clinician or pickup by
patient or representative
* Intervention
QUALITY ASSURANCE
-Should be done and well documented for record
keeping
QUALITY CONTROL
Sample Details
* Proper and detailed request form
* Test scope and limitations
* Patient selection
* Optimal sample collection time
* Acceptable
* Appropriate transport medium
* Sample storage instructions
* Test availability
QUALITY CONTROL
should review and sign the MOP annually,
microbiology dept. supervisor / Bacteriology section
head
RAPID, SENSITIVE and SPECIFIC
Diagnostic test
TEMPERATURE
Incubator and Refrigerator thermometers
* Permanently immersed in –: prevents
temperature fluctuations every time the
door is opened.
glycerol
Equipment (Preventive maintenance)
incubator
daily
Equipment (Preventive maintenance)
anaerobic chamber
daily
Equipment (Preventive maintenance)
BSC
annually, anytime hoods are removed
Equipment (Preventive maintenance)
centrifuge, microscope
every 6mos, or as needed
4x a year
Equipment (Preventive maintenance)
autoclave
each load
Equipment (Preventive maintenance)
balance
anually
Most bacteria grow in pH – while
animal cells thrive in pH 7.2 – 7.4.
6.5 - 7.0
Most bacteria grow in pH 6.5 - 7.0 while
animal cells thrive in pH –
7.2 – 7.4.
Media preparation
plated
1 month
Media preparation
tubed
6 months
reagent - if an organism produces
acetylmethyl carbinol from glucose fermentation
Voges-Proskauer
paper disks impregnated with growth
factors
X and V strips
SUSCEPTIBILITY TESTING
Agar depth
Kirby Bauer method
SUSCEPTIBILITY TESTING
Evaporation
Microtiter dilution
organism:
monitoring of Ca, Mg
P. aeruginosa
organism:
monitoring thymidine
e. faecalis
organism:
for gram + antibiotics
staph aureus
organism:
for gram - antibiotics
e. coli
lowest
concentration of antibiotic that can inhibit organism
MIC- minimum inhibitory concentration
Increasing – concentration
predisposes P. aeruginosa in resistance to
aminoglycosides
calcium and magnesium
Increasing – concentrations will cause false
resistance to drugs such as: sulfonamides,
trimethoprim, trimethoprim sulfamethoxazole
thymidine
a private or
non-profit global biological resource center which
standardize different organisms and strains
ATCC American type culture collection
Organisms should be subcultured – after thawing
to regain its healthy state
twice
media for freezing should
not contain – as the acid produced may kill the
organism in time.
sugars
Preferred media for stock cultures:
optimum media for anaerobes
Chopped meat
Preferred media for stock cultures:
for fungi
Potato dextrose agar
Preferred media for stock cultures:
for
viruses
Fetal bovine serum with 10% dimethyl sulfoxide
states that employee competency must
be determined and verified upon employment.
CLIA 1988
Frozen non fastidious organisms – thawed , recultured
and refrozen every–
5 years
Frozen fastidious organisms – thawed , recultured
and refrozen every–
3 years
sends” blind unknowns”
EQAS or NEQAS
- This serves to establish the accuracy and reproducibility
of laboratory’s everyday performance
PROFICIENCY TESTING
- This serves to establish the accuracy and reproducibility
of laboratory’s everyday performance
PROFICIENCY TESTING
NRL:
CC
Lung center of the Philippines-
NRL:
Drug testing
East avenue
NRL:
Hematology
National kidney transplant institute-
NRL:
HIV/ Aids.
San Lazaro
NRL:
- Bacteriology
- Parasitology
- Mycobacteriology
- Transfusion Transmissible Infections
- SARS-CoV-2 Molecular
Research institute of Tropical Medicine
All QC records should be maintained for at least –except for equipment documentation.
2
years
