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FINAL BACTERIOLOGY > SEMR only: QC and QA > Flashcards

SEMR only: QC and QA Flashcards

1
Q

Pre-analytical phase
* Test requisition
* Processing of test requisition
* Collection of sample
* Sample transport
* Initial processing
* Transport to microbiology section

A

QUALITY ASSURANCE

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2
Q

Analytical phase
* Examination and workup of culture
* Interpretation of results

A

QUALITY ASSURANCE

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3
Q

Post- analytical phase
* Preparation and production of written report
* Distribution of results to the clinician or pickup by
patient or representative
* Intervention

A

QUALITY ASSURANCE

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4
Q

-Should be done and well documented for record
keeping

A

QUALITY CONTROL

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5
Q

Sample Details
* Proper and detailed request form
* Test scope and limitations
* Patient selection
* Optimal sample collection time
* Acceptable
* Appropriate transport medium
* Sample storage instructions
* Test availability

A

QUALITY CONTROL

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6
Q

should review and sign the MOP annually,

A

microbiology dept. supervisor / Bacteriology section
head

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7
Q

RAPID, SENSITIVE and SPECIFIC

A

Diagnostic test

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8
Q

TEMPERATURE
Incubator and Refrigerator thermometers
* Permanently immersed in –: prevents
temperature fluctuations every time the
door is opened.

A

glycerol

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9
Q

Equipment (Preventive maintenance)

incubator

A

daily

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10
Q

Equipment (Preventive maintenance)

anaerobic chamber

A

daily

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11
Q

Equipment (Preventive maintenance)

BSC

A

annually, anytime hoods are removed

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12
Q

Equipment (Preventive maintenance)

centrifuge, microscope

A

every 6mos, or as needed
4x a year

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13
Q

Equipment (Preventive maintenance)

autoclave

A

each load

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14
Q

Equipment (Preventive maintenance)

balance

A

anually

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15
Q

Most bacteria grow in pH – while
animal cells thrive in pH 7.2 – 7.4.

A

6.5 - 7.0

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16
Q

Most bacteria grow in pH 6.5 - 7.0 while
animal cells thrive in pH –

A

7.2 – 7.4.

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17
Q

Media preparation

plated

A

1 month

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18
Q

Media preparation

tubed

A

6 months

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19
Q

reagent - if an organism produces
acetylmethyl carbinol from glucose fermentation

A

Voges-Proskauer

20
Q

paper disks impregnated with growth
factors

A

X and V strips

21
Q

SUSCEPTIBILITY TESTING
Agar depth

A

Kirby Bauer method

22
Q

SUSCEPTIBILITY TESTING
Evaporation

A

Microtiter dilution

23
Q

organism:

monitoring of Ca, Mg

A

P. aeruginosa

24
Q

organism:

monitoring thymidine

A

e. faecalis

25
Q

organism:

for gram + antibiotics

A

staph aureus

26
Q

organism:

for gram - antibiotics

A

e. coli

27
Q

lowest
concentration of antibiotic that can inhibit organism

A

MIC- minimum inhibitory concentration

28
Q

Increasing – concentration
predisposes P. aeruginosa in resistance to
aminoglycosides

A

calcium and magnesium

29
Q

Increasing – concentrations will cause false
resistance to drugs such as: sulfonamides,
trimethoprim, trimethoprim sulfamethoxazole

A

thymidine

30
Q

a private or
non-profit global biological resource center which
standardize different organisms and strains

A

ATCC American type culture collection

31
Q

Organisms should be subcultured – after thawing
to regain its healthy state

A

twice

32
Q

media for freezing should
not contain – as the acid produced may kill the
organism in time.

A

sugars

33
Q

Preferred media for stock cultures:
optimum media for anaerobes

A

Chopped meat

34
Q

Preferred media for stock cultures:
for fungi

A

Potato dextrose agar

35
Q

Preferred media for stock cultures:
for
viruses

A

Fetal bovine serum with 10% dimethyl sulfoxide

36
Q

states that employee competency must
be determined and verified upon employment.

A

CLIA 1988

37
Q

Frozen non fastidious organisms – thawed , recultured
and refrozen every–

A

5 years

38
Q

Frozen fastidious organisms – thawed , recultured
and refrozen every–

A

3 years

39
Q

sends” blind unknowns”

A

EQAS or NEQAS

40
Q
  • This serves to establish the accuracy and reproducibility
    of laboratory’s everyday performance
A

PROFICIENCY TESTING

41
Q
  • This serves to establish the accuracy and reproducibility
    of laboratory’s everyday performance
A

PROFICIENCY TESTING

42
Q

NRL:

CC

A

Lung center of the Philippines-

43
Q

NRL:

Drug testing

A

East avenue

44
Q

NRL:

Hematology

A

National kidney transplant institute-

45
Q

NRL:

HIV/ Aids.

A

San Lazaro

46
Q

NRL:

  • Bacteriology
  • Parasitology
  • Mycobacteriology
  • Transfusion Transmissible Infections
  • SARS-CoV-2 Molecular
A

Research institute of Tropical Medicine

47
Q

All QC records should be maintained for at least –except for equipment documentation.

A

2
years

FINAL BACTERIOLOGY (7 decks)

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