SEMINAL FLUID ANALYSIS Flashcards
Analysis of the specimen cannot begin until liquefaction has occurred
Seminal Fluid Analysis
Color of normal seminal fluid
Grayish white
Color of seminal fluid if there is prolonged abstinence, Medication,
Urine contamination
Yellowish
Color of seminal fluid if there is infection
Increased white turbidity
Color of seminal fluid if there is very low sperm concentration
Clear
Color of seminal fluid if there is blood
Red discoloration
Liquefaction occurs
within 30-60 mins.
Failure of liquefaction to occur within 60 minutes may be caused by a deficiency in ____, and should be ____
prostatic enzymes
reported
Normal volume
2-5 mL
Low volume indicates
Incomplete Collection/Infertility
High volume indicates
Prolonged Abstinence
Can be easily drawn into a pipette and form in droplets that do not appear clumped or stringy
Viscosity
Rating for Viscosity
0 (watery) to 4 (gel-like); low, normal,
or high
Normal pH of seminal fluid
7.2-8.0
Too basic pH indicates
infection
Too acidic pH indicates
increased prostatic fluid, ejaculatory duct obstruction, or poorly developed seminal vesicles
Sperm concentration
> 20,000,000/mL
Sperm count:
> 40,000,000/ejaculate
Diluting fluid in Sperm concentration
Sodium Bicarbonate and Formalin; saline and distilled water
How to determine Sperm concentration in Neubauer
- 5 RBC squares # sperms counted x
1,000,000 = sperms in million/mL - 2 WBC squares # sperms counted x 100,000 = sperms in million/mL
Sperm concentration x volume of specimen
Sperm count
Motility:
> 50% w/in 1 hr
Motility quality:
> 2.0 or a, b, c after 1 hr
T/F
Motility is evaluated by speed only
FALSE
evaluated by BOTH speed and direction
Determines sperm concentration, sperm velocity and trajectory, and morphology
COMPUTER-ASSISTED SEMEN ANALYSIS (CASA)
