Semi conservative replication DNA polymerase Flashcards
In the nucleus, there are free nucleotides which contain three phosphate groups
These nucleotides are known as
Nucleoside triphosphate
The extra phosphates activate the nucleotides, enabling
them to take part in DNA replication
The bases of the free nucleoside triphosphates align with their ……….. on each of the …………. DNA strands
complementary bases on each of the template DNA strands
The enzyme DNA polymerase
synthesises new DNA strands from the two template strands
The enzyme DNA polymerase synthesises new DNA strands from the two template strands
It does this by
catalysing condensation reactions between the deoxyribose sugar and phosphate groups of adjacent nucleotides within the new strands, creating the sugar-phosphate backbone of the new DNA strands
DNA polymerase cleaves (breaks off) the two extra phosphates and uses the energy released to create ………….
the phosphodiester bonds (between adjacent nucleotides)
Hydrogen bonds then form between the complementary base pairs of the
template and new DNA strands