Limiting Factors Affecting Enzymes: Enzyme Concentration

Question 1

The higher the enzyme concentration in a reaction mixture, the ………

and say when this doesn’t apply

Answer 1

the greater the number of active sites available and the greater the likelihood of enzyme-substrate complex formation

As long as there is sufficient substrate available, the initial rate of reaction increases linearly with enzyme concentration

Question 2

If the amount of substrate is limited, at a certain point any further increase in enzyme concentration will

Answer 2

not increase the reaction rate as the amount of substrate becomes a limiting factor