semen analysis

Question 1

top 2 reasons for semen analysis

Answer 1

1- fertility
2- post vasectomy

Question 2

normal semen composed from various structures on male

Answer 2

semen and sperm??

Question 3

baby sperm

Answer 3

spermatozoa: produced in testes under influence of testosterone

Question 4

where does sperm mature

Answer 4

epididymis

Question 5

60% seminal fluid produced in

Answer 5

seminal vesicles with falfin: gives milky look

Question 6

20% of fluid produced in

Answer 6

prostrate

Question 7

prostate produces

Answer 7

acid phosphate and

• helps determine if women are raped
  -liquefaction

Question 8

in seminal vesicles pick up fructose and determine

Answer 8

MADDIE

Question 9

takes sperm how long to mature

Answer 9

3 months

so if testing sperm now it is really the sperm from 3 months ago and can affect results

Question 10

when collecting sperm how long should men not ejaculate for

Answer 10

2-7 days before collection

Question 11

sperm can NOT be collected in anything with a

Answer 11

spermicidal agents - condom

Question 12

sperm should be accessed how long after collection

Answer 12

1-2 hours and be kept warm or else will affect motility

-normally placed in 37 degree incubator for 30 minutes to liquify to analyze

Question 13

if sperm doesn’t liquify in 1 hour

Answer 13

something is wrong; could be producing antibodies against own sperm

Question 14

issues with appearance of sperm for fertility

Answer 14

blood, not viscous enough, yellow (means contaminated with urine)

Question 15

viscosity is measured on rate of

Answer 15

1-4

1= watery
4= doesn’t move

should drip from a pipette

Question 16

normal volume of seminal fluid

Answer 16

2-6 mL

Question 17

normal pH of fluid

Answer 17

7-8

Question 18

if >8 pH

Answer 18

infection’ normally with enterobacteria with conversions of nitrate to nitrites

Question 19

if <7 pH will affect

Answer 19

motility and production of sperm

Question 20

for looking for motility and forward progression of sperm make

Answer 20

wet mount

Question 21

men can have low sperm count but got motile=

Answer 21

good chance of pregnancy

MOTILITY= HUGE

Question 22

how many fields should be estimated for amount of sperm moving

Answer 22

20-25 fields

want to see 50-70% normal motility

Question 23

forward progression

Answer 23

are they moving forward
scale of 1-4

Question 24

normal amount of sperm

Answer 24

20-120 million /mL

Question 25

how is sperm count

Answer 25

on hemocytometer

1. mix with NaOH with some fluid
   2.sit 30 min
2. stops movement of sperm
3. allow to settle before counting

Question 26

how to look at morph for sperm

Answer 26

-spin down liquid so sperm at bottom
-put on slide and stain

normal sized head with tapering tail

70% need to be normal to be considered “normal”

Question 27

<70% normal looking sperm =

Answer 27

infertility

Question 28

what stain does the sperm viability test use

Answer 28

eosin nigrosin

Question 29

what does the sperm viability test determine

Answer 29

what percentage of sperm are viable/ alive

-should coincide with motility

Question 30

colors of sperm viability test

Answer 30

stain will penetrate dead sperm- red
live sperm will be- blueish/white

look in 25 fields to determine overall

Question 31

what percent of sperm do you want to be alive in the sperm viability test

Answer 31

> 50%

Question 32

main sugar sperm uses for energy

Answer 32

fructose

Question 33

we can do a fructose level on

Answer 33

higher % of sperm are dead

-measures amount in seminal fluid

Question 34

if you notice a lot of sperm that are together it could be

Answer 34

male producing antibodies against own sperm

Question 35

how to perform sperm aggulating bodies test

Answer 35

draw male serum and add sperm
-if see agglutination= antibodies

same can be done with female serum

Question 36

what test is done if everything else seems right but you think something is wrong

Answer 36

sperm agglutinating bodies

Question 37

how long does it take post vasectomy to have a sperm count of zero

Answer 37

3 months

gold standard= 3 negative sperm counts mean negative

Question 38

tests we can culture for on seminal fluid

Answer 38

• Neisseria Gonorrhea
• PCR testing for Chlamydia
• PCR for micro plasma hominis
• Serology testing for syphilis