polarizing microscopy Flashcards

1
Q

what is polarizing microscopy

A

allows light to bend in certain direction and gives off colors specific to certain crystals

-uses to diagnose crystals in urine and synovial fluid

-lipids in urine

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2
Q

can bend polarized light

A

anisotropic or birefringent

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3
Q

2 polarizing filters

A

polarizer and analyzer

-put both together and stops direction of light

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4
Q

polarizer

A

placed at bottom of microscope
-forces light to travel in left to right

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5
Q

analyzer

A

placed at head of microscope
-forces light to move north to south

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6
Q

birefringent material

A

-rotate direction of light that passes through
-appears bright against black background

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7
Q

if substance rotates plane of polarized light clockwise

A

positive birefringence

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8
Q

If substance rotates plane of polarized light counterclockwise

A

negative birefringence

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9
Q

to detect another component we add a

A

compensator

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10
Q

what does a compensator do

A

tells us which direction of light is bent in

-placed between analyzer and polarizer

has arrow to indicate direction

aligned 45 degrees

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11
Q

most common compensator

A

red

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12
Q

positive birefringent

A

-blue color when parallel to direction of compensator
-Yellow when perpendicular to compensator

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13
Q

negative birefringent

A

○ Yellow when parallel
-Blue with perpendicular

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14
Q
A
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