Sectioning

Question 1

Aka chuck

Answer 1

Block holder

Question 2

Simplest microtome
Aka cambridge microtome
Invented by Paldwell Trefall

Answer 2

Rocking microtome

Question 3

Routinely used microtome

Invented by Minot

Answer 3

Rotary microtome

Question 4

Invented by Adams

Answer 4

Sliding microtome

Question 5

Type of sliding microtome

Knife held rigidly in clamps

Answer 5

Base sledge

Question 6

More dangerous type of sliding microtome

Answer 6

Standard sliding

Question 7

Invented by Queckett

Answer 7

Freezing microtome

Question 8

Microtome for EM

Answer 8

Ultrathin microtome

Question 9

Cutting sections as thin as ______ in ultrathin microtome

Answer 9

0.5um

Question 10

Special knife in ultrathin microtome
Very sharp knife
Not easily dolled
Very expensive

Answer 10

Diamond knife

Question 11

Length: 25mm

Shortest microtome knife

Answer 11

Plane concave

Question 12

Less concave part of the knife cuts what embedded tissue

Answer 12

Celloidin

Question 13

More concave side is for _________

Answer 13

Paraffin

Question 14

Length: 120mm

Longest microtome knife

Answer 14

Biconcave knife

Question 15

Length: 100mm

Answer 15

Plane wedge

Question 16

=cutting facet

Found in the tapered edge of a knife

Answer 16

Bevel

Question 17

Bevel angle

Answer 17

27-32 degrees

Question 18

Maintains bevel angle

Answer 18

Knife back

Question 19

Cutting angle

Answer 19

15 degrees

Question 20

Clearance angle

Angle formed b/w the surface of the block & the cutting edge of the knife

Answer 20

0-15 degrees

Question 21

Process of removing nicks

Heel to toe

Answer 21

Honing

Question 22

Hone dimension

Answer 22

8 x 3 inch

Question 23

Hone that gives best result

Answer 23

Belgium yellow

Question 24

Hone that gives more polishing effect

Answer 24

Arkansas

Question 25

Hone for badly nicked knives

Answer 25

Fine carborundum

Question 26

Most common lubricant for hones

Answer 26

Soapy water

Question 27

Clean the honed knife w/ __________

Answer 27

Xylene

Question 28

=polishing
Removal of burrs
Toe to heel

Answer 28

Stropping

Question 29

Use paddle strop made of _____________

Answer 29

Horse leather

Question 30

Paraffin sections size

Answer 30

4-6um

Question 31

Celloidin sections size

Answer 31

10-15um

Question 32

Renal biopsy sections size

Answer 32

2um

Question 33

Frozen sections size

Answer 33

4um

Question 34

Point in w/c sections may be cut @ 10um

Answer 34

Dew line

Question 35

Cryostat optimum temp

Answer 35

-18-20C

Question 36

-190C

Most rapid method of freezing

Answer 36

Liquid nitrogen

Question 37

Flotation water bath temp

Answer 37

6-10C below MP of wax

Question 38

Flotation water bath diameter

Answer 38

11inch

Question 39

Flotation water bath height

Answer 39

4inch

Question 40

Flotation water bath capacity

Answer 40

2L

Question 41

Drying oven temp

Answer 41

5C higher than MP of wax

Question 42

Most common tissue adhesive

Answer 42

Albumin

Question 43

Albumin component that serves as adhesive

Answer 43

Egg white

Question 44

Added to ⬆️ viscosity & prevent complete drying

Answer 44

Glycerol

Question 45

Preservative added to prevent growth of molds

Answer 45

Thymol crystals

Question 46

Gelatin concentration

Answer 46

1%

Question 47

Widely used as an adhesive in immunohistochemistry

Answer 47

Poly-l-lysine

Question 48

Freeze drying: preservation by rapid freezing (quenching) @ _______

Answer 48

-160C