Sectioning Flashcards

1
Q

Aka chuck

A

Block holder

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Simplest microtome
Aka cambridge microtome
Invented by Paldwell Trefall

A

Rocking microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Routinely used microtome

Invented by Minot

A

Rotary microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Invented by Adams

A

Sliding microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Type of sliding microtome

Knife held rigidly in clamps

A

Base sledge

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

More dangerous type of sliding microtome

A

Standard sliding

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Invented by Queckett

A

Freezing microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Microtome for EM

A

Ultrathin microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Cutting sections as thin as ______ in ultrathin microtome

A

0.5um

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Special knife in ultrathin microtome
Very sharp knife
Not easily dolled
Very expensive

A

Diamond knife

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Length: 25mm

Shortest microtome knife

A

Plane concave

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Less concave part of the knife cuts what embedded tissue

A

Celloidin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

More concave side is for _________

A

Paraffin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Length: 120mm

Longest microtome knife

A

Biconcave knife

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Length: 100mm

A

Plane wedge

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

=cutting facet

Found in the tapered edge of a knife

A

Bevel

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Bevel angle

A

27-32 degrees

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Maintains bevel angle

A

Knife back

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Cutting angle

A

15 degrees

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Clearance angle

Angle formed b/w the surface of the block & the cutting edge of the knife

A

0-15 degrees

21
Q

Process of removing nicks

Heel to toe

A

Honing

22
Q

Hone dimension

A

8 x 3 inch

23
Q

Hone that gives best result

A

Belgium yellow

24
Q

Hone that gives more polishing effect

A

Arkansas

25
Q

Hone for badly nicked knives

A

Fine carborundum

26
Q

Most common lubricant for hones

A

Soapy water

27
Q

Clean the honed knife w/ __________

A

Xylene

28
Q

=polishing
Removal of burrs
Toe to heel

A

Stropping

29
Q

Use paddle strop made of _____________

A

Horse leather

30
Q

Paraffin sections size

A

4-6um

31
Q

Celloidin sections size

A

10-15um

32
Q

Renal biopsy sections size

A

2um

33
Q

Frozen sections size

A

4um

34
Q

Point in w/c sections may be cut @ 10um

A

Dew line

35
Q

Cryostat optimum temp

A

-18-20C

36
Q

-190C

Most rapid method of freezing

A

Liquid nitrogen

37
Q

Flotation water bath temp

A

6-10C below MP of wax

38
Q

Flotation water bath diameter

A

11inch

39
Q

Flotation water bath height

A

4inch

40
Q

Flotation water bath capacity

A

2L

41
Q

Drying oven temp

A

5C higher than MP of wax

42
Q

Most common tissue adhesive

A

Albumin

43
Q

Albumin component that serves as adhesive

A

Egg white

44
Q

Added to ⬆️ viscosity & prevent complete drying

A

Glycerol

45
Q

Preservative added to prevent growth of molds

A

Thymol crystals

46
Q

Gelatin concentration

A

1%

47
Q

Widely used as an adhesive in immunohistochemistry

A

Poly-l-lysine

48
Q

Freeze drying: preservation by rapid freezing (quenching) @ _______

A

-160C