Sectioning Flashcards
Aka chuck
Block holder
Simplest microtome
Aka cambridge microtome
Invented by Paldwell Trefall
Rocking microtome
Routinely used microtome
Invented by Minot
Rotary microtome
Invented by Adams
Sliding microtome
Type of sliding microtome
Knife held rigidly in clamps
Base sledge
More dangerous type of sliding microtome
Standard sliding
Invented by Queckett
Freezing microtome
Microtome for EM
Ultrathin microtome
Cutting sections as thin as ______ in ultrathin microtome
0.5um
Special knife in ultrathin microtome
Very sharp knife
Not easily dolled
Very expensive
Diamond knife
Length: 25mm
Shortest microtome knife
Plane concave
Less concave part of the knife cuts what embedded tissue
Celloidin
More concave side is for _________
Paraffin
Length: 120mm
Longest microtome knife
Biconcave knife
Length: 100mm
Plane wedge
=cutting facet
Found in the tapered edge of a knife
Bevel
Bevel angle
27-32 degrees
Maintains bevel angle
Knife back
Cutting angle
15 degrees
Clearance angle
Angle formed b/w the surface of the block & the cutting edge of the knife
0-15 degrees
Process of removing nicks
Heel to toe
Honing
Hone dimension
8 x 3 inch
Hone that gives best result
Belgium yellow
Hone that gives more polishing effect
Arkansas
Hone for badly nicked knives
Fine carborundum
Most common lubricant for hones
Soapy water
Clean the honed knife w/ __________
Xylene
=polishing
Removal of burrs
Toe to heel
Stropping
Use paddle strop made of _____________
Horse leather
Paraffin sections size
4-6um
Celloidin sections size
10-15um
Renal biopsy sections size
2um
Frozen sections size
4um
Point in w/c sections may be cut @ 10um
Dew line
Cryostat optimum temp
-18-20C
-190C
Most rapid method of freezing
Liquid nitrogen
Flotation water bath temp
6-10C below MP of wax
Flotation water bath diameter
11inch
Flotation water bath height
4inch
Flotation water bath capacity
2L
Drying oven temp
5C higher than MP of wax
Most common tissue adhesive
Albumin
Albumin component that serves as adhesive
Egg white
Added to ⬆️ viscosity & prevent complete drying
Glycerol
Preservative added to prevent growth of molds
Thymol crystals
Gelatin concentration
1%
Widely used as an adhesive in immunohistochemistry
Poly-l-lysine
Freeze drying: preservation by rapid freezing (quenching) @ _______
-160C