Clearing

Question 1

Aka xylol, colorless clearing agent, most commonly used in routine procedures

Answer 1

Xylene

Question 2

Xylene clearing time

Answer 2

15-30 mins to 1 hr

Question 3

Xylene tissue section thickness

Answer 3

<5mm

Question 4

Xylene turns _____ when tissues are incompletely dehydrated

Answer 4

Milky

Question 5

Aka toluol, substitute for xylene/benzene

Answer 5

Toluene

Question 6

Toluene clearing time

Answer 6

1-2 hrs

Question 7

Recommended for urgent biopsies & for routine purposes

Answer 7

Benzene

Question 8

Benzene clearing time

Answer 8

15-60 mins

Question 9

Recommended for tough tissues/large tissues
tissues don’t become transluscent
toxic to liver

Answer 9

Chloroform

Question 10

Recommended for CNS tissues & cytology such as smooth muscles & skin
no tissue distortion even if left in oil indefinitely
tends to become too milky upon prolonged storage

Answer 10

Cedarwood oil

Question 11

Cedarwood oil clearing time

Answer 11

2-3 days

Question 12

For clearing embryos, insects and delicate specimens

Answer 12

Aniline oil

Question 13

Becomes adulterated

easily contaminated

Answer 13

Clove oil

Question 14

For double embedding techniques

Answer 14

Methyl benzoate

Methyl salicylate