Impregnation

Question 1

Simplest, most common & best embedding medium

Answer 1

Paraffin

Question 2

Paraffin MP

Answer 2

56C

Question 3

Histopath techniques performed in automatic processing

Answer 3

Fixation dehydration clearing infiltration

Question 4

# of stations in automatic tissue processor
(10 1L containers & 2 wax baths)

Answer 4

12

Question 5

Rgt in station 1

Answer 5

Formalin

Question 6

Rgt in last station

Answer 6

Paraffin

Question 7

Automatic processing main adv

Answer 7

Constant agitation

Question 8

Fresh wax must be filtered by a coarse filter using __________

Answer 8

Green’s number 904

Question 9

Paraffin wax can only be used ___

Answer 9

2x

Question 10

Water in the wax must be removed by _________

Answer 10

Heating @ 100-105C

Question 11

Mixture of paraffin & synthetic plaster polymer

Answer 11

Paraplast

Question 12

Paraplast MP

Answer 12

56-57C

Question 13

Similar to paraplast

MP: 56-58C

Answer 13

Embeddol

Question 14

Semisynthetic that is used in embedding eyes

Answer 14

Bioloid

Question 15

Contains rubber

Answer 15

Tissue mat

Question 16

MP: 46-48

Harder than paraffin

Answer 16

Ester wax

Question 17

Ester wax is soluble in __________

Answer 17

95% ethanol

Question 18

Ester wax is sectioned using ___________________

Answer 18

Sliding or sledge microtome

Question 19

Most common water soluble wax

Answer 19

Carbowax

Question 20

Aka: collodion

Answer 20

Celloidin

Question 21

Wet celloidin is for _______________

Answer 21

Bones
Brain sections
Teeth

Question 22

Storage for wet celloidin

Answer 22

70% alcohol

Question 23

Dry celloidin is for _____________

Answer 23

Whole eye sections

Question 24

Dry celloidin storage

Chloroform + cedarwood oil

Answer 24

Gilson’s mixture

Question 25

Plasticizer added to prevent tissue cracking

Answer 25

Castor oil or oleum ricini

Question 26

Gelatin volume should be at least ______ the volume of tissue

Answer 26

25x