SECTIONING Flashcards
correct shape of the tissue block
4-sided prism/truncated pyramid
recommended thickness for routine tissue microtomy
4-6 u
recommended thickness for frozen sections
10-15 u
recommended thickness for electron microscopy
0.5 u
kinds of microtome:
simplest but difficult to reorient the block
rocking microtome
kinds of microtome:
most common microtome used for paraffin embedded blocks
rotary/minot microtome
kinds of microtome:
most dangerous type due to movable exposed knife
sliding microtome
type of sliding microtome:
knife: stationary
block: moving
for all forms of media
base sledge
type of sliding microtome:
knife: moving
block: stationary
standard sliding microtome
kinds of microtome:
used for unfixed, unfrozen specimen for enzyme demonstration
vibrotome
kinds of microtome:
for electron microscopy
ultrathin microtome
what knives are used for electron microscopy
diamond knives or broken plate glass
fixative and embedding media for ultrathin microtome
fixative: osmium tetroxide
embedding media: plastic
temperature of floatation bath
45-50 C or 6-10 C below melting point
color of floatation bath
black