SECTIONING Flashcards

1
Q

correct shape of the tissue block

A

4-sided prism/truncated pyramid

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2
Q

recommended thickness for routine tissue microtomy

A

4-6 u

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3
Q

recommended thickness for frozen sections

A

10-15 u

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4
Q

recommended thickness for electron microscopy

A

0.5 u

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5
Q

kinds of microtome:

simplest but difficult to reorient the block

A

rocking microtome

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6
Q

kinds of microtome:

most common microtome used for paraffin embedded blocks

A

rotary/minot microtome

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7
Q

kinds of microtome:

most dangerous type due to movable exposed knife

A

sliding microtome

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8
Q

type of sliding microtome:

knife: stationary
block: moving
for all forms of media

A

base sledge

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9
Q

type of sliding microtome:

knife: moving
block: stationary

A

standard sliding microtome

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10
Q

kinds of microtome:

used for unfixed, unfrozen specimen for enzyme demonstration

A

vibrotome

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11
Q

kinds of microtome:

for electron microscopy

A

ultrathin microtome

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12
Q

what knives are used for electron microscopy

A

diamond knives or broken plate glass

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13
Q

fixative and embedding media for ultrathin microtome

A

fixative: osmium tetroxide

embedding media: plastic

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14
Q

temperature of floatation bath

A

45-50 C or 6-10 C below melting point

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15
Q

color of floatation bath

A

black

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