INFILTRATION Flashcards
replacing the clearing agent with the infiltrating media
infiltration
most common, simplest, and best embedding/infiltrating medium
paraffin wax
paraffin wax is not recommended for:
fatty tissues
scientist who introduced paraffin wax embedding
Butschlii
temperature of paraffin oven
55-60 C or 2-5 C above melting point
substitute for paraffin wax:
more elastic and resilient than paraffin
paraplast
paraplast melting point
56-57 C
substitute for paraffin wax:
for embedding eyes
bioloid
substitute for paraffin wax:
- not soluble in water, soluble in 95% ethanol and other clearing agents
- clearing is not needed
ester wax
microtome used for ester wax
sliding/sledge microtome
most commonly used water soluble wax:
carbowax
dehydration is not needed anymore if you used carbowax. t or f?
true
recommended amount/ratio for impregnation
25x the tissue volume or 20:1
4 types of infiltrating and embedding media
- paraffin
- celloidin
- gelatin
- plastic
substitute for paraffin wax:
recommended for large dense tissue blocks (bones and brain)
paraplast
substitute for paraffin wax:
less brittle and less compressible than paraplast
embeddol
substitute for paraffin wax:
suitable for histochemical studies
water-soluble waxes
purified form of nitrocellulose, suitable for specimens with large hollow cavities, hard and dense tissues, whole embryo
celloidin
celloidin that is recommended for bones, teeth, brain and organ sections
wet celloidin
celloidin that is recommended for whole eye sections
dry celloidin
embedding medium for delicate specimens and frozen
sections because it prevents fragmentation of tough and friable tissues when frozen sections are cut
gelatin
recommended for electron microscopy
plastic/resin
3 class of plastic media
glycerol
bisphenol A
cyclohexene dioxide
