INFILTRATION

Question 1

replacing the clearing agent with the infiltrating media

Answer 1

infiltration

Question 2

most common, simplest, and best embedding/infiltrating medium

Answer 2

paraffin wax

Question 3

paraffin wax is not recommended for:

Answer 3

fatty tissues

Question 4

scientist who introduced paraffin wax embedding

Answer 4

Butschlii

Question 5

temperature of paraffin oven

Answer 5

55-60 C or 2-5 C above melting point

Question 6

substitute for paraffin wax:

more elastic and resilient than paraffin

Answer 6

paraplast

Question 7

paraplast melting point

Answer 7

56-57 C

Question 8

substitute for paraffin wax:

for embedding eyes

Answer 8

bioloid

Question 9

substitute for paraffin wax:

• not soluble in water, soluble in 95% ethanol and other clearing agents
• clearing is not needed

Answer 9

ester wax

Question 10

microtome used for ester wax

Answer 10

sliding/sledge microtome

Question 11

most commonly used water soluble wax:

Answer 11

carbowax

Question 12

dehydration is not needed anymore if you used carbowax. t or f?

Answer 12

true

Question 13

recommended amount/ratio for impregnation

Answer 13

25x the tissue volume or 20:1

Question 14

4 types of infiltrating and embedding media

Answer 14

1. paraffin
2. celloidin
3. gelatin
4. plastic

Question 15

substitute for paraffin wax:

recommended for large dense tissue blocks (bones and brain)

Answer 15

paraplast

Question 16

substitute for paraffin wax:

less brittle and less compressible than paraplast

Answer 16

embeddol

Question 17

substitute for paraffin wax:

suitable for histochemical studies

Answer 17

water-soluble waxes

Question 18

purified form of nitrocellulose, suitable for specimens with large hollow cavities, hard and dense tissues, whole embryo

Answer 18

celloidin

Question 19

celloidin that is recommended for bones, teeth, brain and organ sections

Answer 19

wet celloidin

Question 20

celloidin that is recommended for whole eye sections

Answer 20

dry celloidin

Question 21

embedding medium for delicate specimens and frozen

sections because it prevents fragmentation of tough and friable tissues when frozen sections are cut

Answer 21

gelatin

Question 22

recommended for electron microscopy

Answer 22

plastic/resin

Question 23

3 class of plastic media

Answer 23

glycerol
bisphenol A
cyclohexene dioxide