DEHYDRATION

Question 1

the process of removing intercellular and extracellular water from the tissue

Answer 1

dehydration

Question 2

ratio of dehydrating agent to tissue

Answer 2

10:1

Question 3

dehydration is done at what temp?

Answer 3

room temp

Question 4

routine tissue alcohol grades:

Answer 4

70 - 95 - 100

Question 5

delicate tissue alcohol grades:

Answer 5

30 onwards

Question 6

alcohol conc above 80% will make tissues:

Answer 6

hard, brittle, and difficult to cut

Question 7

alcohol conc below 70% will make tissues:

Answer 7

wither or macerated

Question 8

this reagent ensures complete dehydration and removes water from the dehydrating fluid

Answer 8

anhydrous copper sulfate

Question 9

what does blue discoloration of copper sulfate means?

Answer 9

there is still presence of water

Question 10

BEST dehydrating agent

Answer 10

ethanol

Question 11

dehydrating agent for blood and tissue films

Answer 11

methanol

Question 12

dehydrating agent for plant and animal micro-techs

sweet and rancid odor

Answer 12

butanol

Question 13

universal solvent

Answer 13

tertiary butanol

Question 14

best clearing agent for microwave tech

Answer 14

isopropanol

Question 15

dehydrating agent used for most urgent biopsies; also a fixative

Answer 15

acetones

Question 16

the excellent universal solvent

Answer 16

dioxane

Question 17

method that uses pure dioxane and paraffin wax

Answer 17

graupners

Question 18

method that uses dioxane quicklime

Answer 18

weisebergers

Question 19

dehydrating agent that can be stored for months with no hardening or distortion
combustible at 110-120 F
highly toxic

Answer 19

cellosolve

Question 20

dehydrating agent that removes water very readily

Answer 20

triethyl phosphate

Question 21

dehydrating agent that does not dissolve aniline dyes

have ether like odor

Answer 21

tetrahydrofuran

Question 22

3 universal solvents

Answer 22

tertiary butanol
dioxane
tetrahydrofuran

Question 23

typical dehydration sequence for <4mm thick sections

Answer 23

70% ethanol - 15 min
90% ethanol - 15 min
100% ethanol - 15 min 
100% ethanol - 15 min
100% ethanol - 30 min
100% ethanol - 45 min

Question 24

additives:

acts as tissue softeners

Answer 24

4% phenol + each 95% ethanol baths