section 20 Flashcards
cytochemical stains
1
Q
what is the purpose of using cytochemical stains
A
to differentiate between blast lineages (myelo/mono)
2
Q
myeloperoxidase stain
A
stains peroxidase granules in myleo/mono blasts
3
Q
what else are specific esterases called
A
napthol AS-D chloroacetate esterase
4
Q
what is the purpose of SPE
A
stains myeloblasts
5
Q
what other compound is used with non specific esterases and why
A
NaF to distinguish between myelo and mono lineage
- monocytic lineage will remain positive with NaF
6
Q
LAP
A
stains secondary granules in neutrophils to be quantified 0-4
7
Q
when is LAP used
A
to determine shift to the left vs malignancy
- malignancy = low
- leukemoid = high
8
Q
what is the purpose of TRAP staining
A
to differentiate hairy cells from other WBC by presence of isoenzyme 5
