Section 2 - Transport across cell membranes Flashcards

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1
Q

What is the function of glycoproteins in the cell-surface membrane?
(5 things)

A
  1. Receptors
  2. Antigens
  3. Cell signalling
  4. Receptor for hormone
  5. Cell adhesion
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2
Q

How can small, non polar substances move through membrane?

A

Pass through phospholipid bilayer

By simple diffusion

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3
Q

How can large substances move through membrane?

A

Transport
Carrier proteins
Endocytosis

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4
Q

How can polar substances move through membrane?

A

Facilitated diffusion
Channel and carrier proteins

Active transport - use of ATP

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5
Q

What is the role of membranes within cells?

A

Compartmentalisation

Reactions take place on membranes

Enzymes attached to membrane

Nuclear pore permits RNA to leave

Increases surface area of organelle

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6
Q

Why are phospholipids in the cell-surface orientated in a specific way?

A

Hydrophilic heads attracted to water

Hydrophobic tail repelled by water so they point into centre.

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7
Q

What does cholesterol within the cell-surface membrane do?

A

Add strength to the membrane
Reduce movement
Reduces fluidity

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8
Q

What is the process of co-transport?

A
  1. Sodium ions actively transported out of epithelial cells by sodium-potassium pump
  2. Low concentration of sodium ions in epithelial cell, so sodium ions diffuses down concentration gradient bringing glucose/ amino acid with it
  3. Glucose passes into blood plasma by facilitated diffusion
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9
Q

How do bacteria divide?

A

Binary fission

Replication of DNA
Division of cytoplasm

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10
Q

How does the phospholipid bilayer affect entry and exit of substances into and out of a cell?

A

Allows movement of lipid soluble/ non-polar molecules

Prevents movement of water soluble molecules

Large molecules do not move through

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11
Q

What are the functions of the cell-surface membrane?

A
Providing partially permeable barrier
Compartmentalisation
Transport of solutes
Signal transduction
Cell to cell recognition
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12
Q

What are the 2 organelles without a membrane?

A

Ribosome

Centriole

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13
Q

How many nanometres wide are membranes?

A

7 nm

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14
Q

Why is the cell-surface membrane also known as the fluid mosaic model?

A

Fluid: individual phospholipids and proteins can move around freely within their monolayer

Mosaic: pattern produced by scattered protein molecules when the membrane is viewed from above

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15
Q

What is the difference between intrinsic and extrinsic proteins in the membrane?

A

Intrinsic proteins: span the bilayer and form channel/ carrier proteins

Extrinsic proteins: Found on one side of bilayer, involved in cell recognition or act to stabilise membrane

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16
Q

Why are phospholipids important in the membrane?

A

Form sheets (bilayer)

Hydrophilic heads attract water

Hydrophobic tails repel water

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17
Q

What is the function of the phospholipid in the membrane?

A

Allow lipid-soluble substances to enter and leave the cell

Prevent water-soluble substances entering and leaving the cell

Make the membrane flexible and self-sealing

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18
Q

What is the function of proteins in the membrane?

A

Provide structural support

Act as a channels transporting water-soluble substances across

Allow active transport through carrier proteins

Form cell-surface receptors for identifying cells

Acts as receptors

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19
Q

What are the functions of cholesterol in the membrane?

A

Reduce lateral movement of other molecules including phospholipids

Make membrane less fluid at high temperatures

Prevent leakage of water and dissolved ions from the cell

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20
Q

Why is cholesterol important in the membrane?

A

Add strength

Limit movement of fatty acid tails by pulling them together

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21
Q

What is the function of glycolipids in the membrane?

A

Act as a recognition site

Help to maintain stability of the membrane

Help cells to attach forming tissues

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22
Q

What is the function of glycoproteins in the membrane?

A

Help cells to attach forming tissues

Allows cells to recognise one another

Acts as a recognition site

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23
Q

What are the glycolipids in the membrane made of?

A

Carbohydrate covalently bonded to a lipid

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24
Q

What are the glycoproteins in the membrane made of?

A

Carbohydrate attached to extrinsic protein

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25
Q

What is fluidity of the membrane controlled by?

A

Amount of cholesterol

Length of fatty acid
(shorter increases fluidity)

Proportion of saturated and unsaturated fatty acids
(Saturated decreases fluidity)

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26
Q

Why do most molecules not freely diffuse across the membrane?

A

Not soluble in lipids

Too large to pass through

Of same charge as protein channel

Electrically charged (polar)

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27
Q

What is the function of the membrane in lysosmes?

A

Isolated harmful substances to the cell

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28
Q

What is the function of the membrane in mitochondria/ chloroplast?

A

Controls reaction rate
Increases surface area
Controls rate substances enter

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29
Q

What is the function of the membrane in nucleus?

A

Forms separate compartment for cells

Allows RNA out in the pores

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30
Q

What is the function of the membrane in vesicles?

A

Allows substances in and out of cell

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31
Q

What is the function of the membrane in cell surface?

A

Take up fluid and form membrane-bound vesicles

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32
Q

What is the overall function of the cell-surface membrane?

A

To control the movement of substances in and out of the cell

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33
Q

Which end of the phospholipid lies towards the inside of the cell-surface membrane?

A

Hydrophobic tail

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34
Q

What 2 properties should a drug possess if it is to enter a cell rapidly?

A

Lipid soluble
Small in size
Have no electrical charge
Opposite charge of protein channel

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35
Q

What are the features of facilitated diffusion?

A

Passive process
Down concentration gradient
No energy is requires

Requires channel or carrier proteins to pass through membrane

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36
Q

What are channel protiens?

A

Form water-filled hydrophilic channels across membrane

Allow specific water-soluble ions to pass through

Channels are selective, each opening to specific ion

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37
Q

What are carrier proteins?

A

Molecule fits into proteins

Protein changes shape and molecule passes through

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38
Q

What is osmosis?

A

Net movement of water molecules from a region of higher water potential to a region of lower water potential across a partially permeable membrane.

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39
Q

What are 3 factors that affect the rate of diffusion?

A

Concentration gradient

Diffusion area

Thickness of exchange surface

Temperature

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40
Q

What is the difference between facilitated and simple diffusion?

A

Facilitated only occurs at channels on the membrane where there are specific protein carrier molecules

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41
Q

Why is facilitated diffusion a passive process?

A

No ATP from respiration used in the process

42
Q

Why do glucose molecules not easily pass through the phospholipid bilayer?

A

Only lipid-soluble substances diffuse across the phospholipid bilayer

43
Q

What are 2 structures of the membrane that would increase the rate at which glucose is transported into a cell?

A

Increase in surface area

More proteins with pores

44
Q

What is osmosis?

A

Net movement of water molecules from a region of higher water potential to a region of lower water potential across a partially permeable membrane.

45
Q

If you have a high water potential, you have a…

A

Low solute concentration

46
Q

If you have a low water concentration, you have a…

A

High solute concentration

47
Q

Where would water move with 3 water potentials of

  • 400
  • 600
  • 200
A
  • 200 into -400 & -600

- 400 into -600

48
Q

What does hypotonic mean?

A

Region of higher water potential (lower solute)

49
Q

What does hypertonic mean?

A

Region of lower water potential (higher solute)

50
Q

What does isotonic mean?

A

Region where equal water potential on either side of membrane

51
Q

What would happen to an animal cell if it was placed in a hypotonic cell?

A

Water potential of cell lower than external

Water enters cell by osmosis and bursts

52
Q

What would happen to an animal cell if it was placed in a hypertonic cell?

A

Water potential of cell higher than external

Water leaves cell by osmosis and shrinks

53
Q

What would happen to an animal cell if it was placed in a isotonic cell?

A

Water potential of cell same as external

Water enter/ leaves as same rate = dynamic equilibrium

54
Q

What would happen to a plant cell if it was placed in a hypotonic cell?

A

As water enter turgor pressure increases, resists further entry

Cell is turgid

55
Q

What would happen to a plant cell if it was placed in a hypertonic cell?

A

As water leaves, membrane pulls away from cell (plasmolysis)

Cell is flaccid

56
Q

What would happen to a plant cell if it was placed in a isotonic cell?

A

No net movement of water

Cells stays the same

57
Q

What would happen to a plant cell if it was placed in a isotonic cell?

A

No net movement of water

Cells stays the same

58
Q

What is a selectively permeable membrane?

A

Membrane permeable to water molecules but not to larger molecules

59
Q

Under standard conditions of pressure and temperature, what is the water potential of pure water?

A

Zero

60
Q

What is active transport?

A

The movement of molecules into or out of a cell from a region of lower concentration to a region of higher concentration

61
Q

What 2 things does active transport require?

A

ATP

Carrier proteins

62
Q

What is the process of active transport?

A
  1. Molecule binds to complimentary site on carrier protein
  2. ATP is hydrolysed to ADP and carrier protein changes shape
  3. Molecule is transported across membrane
  4. One way flow as membrane can only fit into carrier protein on one side of membrane
63
Q

What is the difference between endocytosis and exocytosis?

A

Endocytosis: moving large amounts in
Exocytosis: moving large amount out

64
Q

How does active transport differ from passive forms of transport?

A

ATP is needed

Against concentration gradient

Carrier proteins act as ‘pumps’

Selective process

65
Q

What is one difference and one similarity between active transport and facilitated diffusion?

A

Similarity: both use carrier protein in plasma membrane

Difference: active transport requires ATP/ occurs against concentration gradient

66
Q

Why are many mitochondria present in cells that carry out active transport?

A

Mitochondria supply ATP in cells which is required for active transport to occur

67
Q

What is the process of co-transport?

A
  1. Na ions actively transported out epithelial cell by sodium-potassium pump into blood
  2. Na ions diffuse into epithelial cell down conc. gradient through protein carrier and carries glucose with it
  3. Glucose passes into blood by facilitated diffusion using carrier protein
68
Q

How is a low concentration of sodium ions in the cell ensured in active transport?

A

Sodium potassium ion ATPase pump, pumps out 3 sodium in exchange for 2 potassium ions

69
Q

What are the 3 types of co-transporter proteins?

A
Uniport = single
Symport = 2 in same direction
Antiport = 2 in alternate direction
70
Q

What are 3 ways the rate of movement across membrane can be increased?

A

Increasing concentration gradient

Increasing surface area

Increasing density of protein channel

71
Q

Why is the term co-transport use to describe the transport of glucose into cells?

A

As glucose molecules and sodium ions move into the cells coupled together

72
Q

What are 2 similarities between the movement of substances in diffusion and osmosis?

A

Both move down concentration gradient
Passive process
Don’t use energy/ ATP

73
Q

Where in plants can cells undergoing mitosis be found?

A

Shoot and root tips

74
Q

What is the mitotic index?

A

Ratio of cells undergoing mitosis to the total number of cells in sample

75
Q

How do you work out mitotic index?

A

Mitotic index=

number of cells with visible chromosomes / number of cells in sample

76
Q

What are the hazards and precautions needed to be taken in the mitotic index practical?

A

HCl = corrosive, avoid contact with skin, eye protection

Blue stain = irritant, avoid contact with skin

Scalpel = cut away from fingers

77
Q

What is the purpose of calibration curves?

A

Used to determine concentration of unknown sample, comparing it to set of standard values with known concentrations

78
Q

How is a calibration curve used to find concentration of plant tissue?

A

Plot calibration curve of percentage change in mass against concentration

Find x-intercept where plant tissue is isotonic to sucrose solution

79
Q

How would you prepare a root tip squash?

A
  1. Use mounted needle to transfer root tips to clean microscope slide
  2. Add drop of water and spread root tips with mounted needle
    =no overlap
  3. Place another microscope slide on top, at right angles, minimising air bubbles
  4. Press slides together to squash root tips and spread them out
80
Q

What occurs when plant tissue is placed in a hypotonic solution?

A

Water moves into plant tissue by osmosis, plant tissue increases in mass

81
Q

What occurs when plant tissue is placed in hypertonic soltuion?

A

Water moves out of plant tissue by osmosis, plant tissue decreases in mass

82
Q

Why are potato discs left in solution for 20 minutes?

A

To allow time for osmosis until plant tissue reaches equilibrium with its surrounding solution

83
Q

What is water potential determined by?

A

The concentration of solutes

84
Q

Why is percentage change in mass of potato used rather than actual change in mass?

A

Potato chips may not all have same starting mass

Percentage change allows comparison

85
Q

What is the procedure of investigating osmosis using potato tissue?

A
  1. Make simple dilution of 1M sucrose to produce 5 concentration. Add 5cm to 5 different test tubes.
  2. Cut potato into equal sized chips and weigh
  3. Place chip in each test tube and leave for 20 minutes
  4. Take out and dab excess water and weigh again
  5. Calculate percentage change in mass
86
Q

What is indicated by the x-intercept of the calibration curve?

A

The concentration that is isotonic to solution tested

87
Q

What does the mass of potato change after 20 minutes?

A

Potato chips with concentration lower than sucrose solution lose mass as there is not movement of water out of cell

Potato chips with concentration higher than sucrose solution gain mass as there is net movement of water into cells

88
Q

Why are potato chips dabbed dry after removing from the sucrose solution?

A

To remove any excess water clinging to its surface

89
Q

What are the controlled variables of the potato practical?

A

Volume of sucrose solution
Size of potato chips
Length of time left in solution

90
Q

What are 2 factors that affect permeability of cell membranes?

A

Temperature

Concentration of solvents

91
Q

How is beetroot used to measure permeability of cell membranes?

A

Higher permeability, more red pigment leaks into solution.

Colorimeter used to determine absorbance

92
Q

What is the process of investigating the effect of temperature on permeability of cell membrane?

A
  1. Cut beetroot into 6 identical cubes
  2. Place each cube in different test tube with equal volume of distilled water
  3. Place each test tube into water baths at various temperatures
  4. Filter each solution into cuvette and measure absorbance using colorimeter
93
Q

What is the effect on temperature on membrane permeability?

A

Increasing temperature results in increase membrane permeability

94
Q

What is the effect of ethanol concentration on membrane permeability?

A

Increasing ethanol concentration leads to increased membrane permeability

95
Q

Why would placing potato in ethanol stop osmosis?

A

Ethanol dissolved the phospholipids

96
Q

Why would boiling a potato piece stop osmosis?

A

Boiling disrupts membrane by denaturing the proteins

97
Q

What is the sequence of events following the production of extracellular proteins that leads to their release from the cell?

A

Moved to golgi apparatus where they’re processed

Into vesicles which fuse with membrane

Exocytosis

98
Q

What are some of the roles of membranes within cells?

A

Compartmentalisation

Nuclear pore permits RNA to leave nucleus

Attachment of ribosomes

99
Q

How can a carrier protein move glucose across a membrane by facilitated diffusion?

A

Glucose molecule attaches to carrier protein

Protein changes shape and is released on the opposite side

100
Q

Describe a method a student could use to prepare colour standards without using a colorimeter?

A

Use known concentration of blueberry juice

Prepare dilution series

Use colour standards for comparisons