Section 2- Cell structure Flashcards
What is resolution?
The minimum distance apart 2 objects can be in order for the to appear as separate items.
What is magnification?
How many times bigger the image is when compared to the object.
How do you convert from millimetres to micrometres?
Multiply by 1000
What of these organelles does a virus have?
- Cell-surface membrane
- Nucleus
- Cytoplasm
- Capsid
Only capsid
no cell-surface membrane, nucleus, cytoplasm
Why can mitochondria look different to one another?
Very in shape
Cut in different planes
What are 3 reasons why mitosis is important to organisms?
Asexual reproduction
Growth in organisms
Repair in organisms
What are 3 ways DNA in chloroplast is different to DNA in nucleus?
Fewer genes
Shorter in length
No introns
Circular not linear
Why are organelle structures not visible in optical microscopes?
Resolution is not high enough
Light microscope have longer wavelength
What organelle is found in both chloroplast and prokaryotic cell?
Ribosomes
What type of cell wall is found in plants, algae, fungi and prokaryotes?
(cellulose, murein, chitin)
Plants: cellulose
Algae: cellulose
Fungi: chitin
Prokaryotes: murein
What is the structure of a prokaryote?
Cell wall
Capsule: protects bacteria form other cells
Cell-surface membrane
Circular DNA
Plasmid: has genes that may aid survival of bacteria
What is the difference between prokaryotic and eukaryotic cells?
Eukaryotic cells: Distinct nucleus with envelope DNA associated with proteins No plasmids, DNA is linear Membrane bound organelles Ribosomes are larger
Prokaryotic: No nucleus Some DNA in plasmids No membrane-bound organelles Ribosomes are smaller Cell wall made of murein Have capsule
What 2 types of molecules are ribosomes made from?
Ribosomal RNA
Protein
What is the molecule found in the cell walls of bacterial cells?
Murein
Why can light microscopes only distinguish between 2 objects if they are 0.2um apart?
Due to the relatively long wavelength of light.
What are the evaluative points for a light microscope?
Low resolution
- Due to long wavelength of light
- Limits magnification
Able to view living specimen
Able to see colour (with stain)
Simple preparation
Artefacts are rare
What are the evaluative points of an electron microscope?
Uses beam of electrons to produce an image
High resolution
-Short wavelength of electrons
Sample must be dead
-Chamber is a vacuum
Image produced is black and white
Preparation is complicated
Artefacts are common
What are the 2 types of electron microscope?
Transmission electron microscope
Scanning electron microscope
What is a transmission electron microscope?
Passes beam of electrons through a very thin specimen
Parts of the specimen allow electrons to pass through and so appear bright
What are the limitations of a transmission electron microscope?
Difficult preparation limits resolution
System is a vacuum = dead specimen
Image is 2D and black and white
May contain artefacts
What is a scanning electron microscope?
Produced a 3D image of the surface of the object
Resolution isn’t as high
3D image can be built by computer analysis of the pattern and secondary electrons
What are the 2 processes of cell fractionation?
Homogenisation
Ultracentrifugation
What 3 kind of solutions does a tissue need to be placed in before cell fractionation?
Cold
Isotonic
Buffer
What does a tissue need to be placed in a cold solution before cell fractionation?
Reduces activity of enzymes which may break down organelles
Why does a tissue need to be placed in a isotonic solution before cell fractionation?
Prevent shrining and bursting due to osmosis
What does a tissue need to be placed in a buffer solution before cell fractionation?
To maintain a constant pH, avoiding damage to organelles
Why is a tissue firstly placed in a homogeniser and filtered before going in a centrifuge?
Releases organelles from the cells
Filtered to remove any whole cells and large debris (cell wall)
What is the process of cell fractionation?
- Tissue placed in cold, isotonic, buffer solution.
- Homogenisation
- Filter homogenate
- Centrifuge homogenate and collect sediment
- Spin at faster speeds and collect sediment
What is the order of organelles in the sediment of an animal cell when centrifuged?
Nuclei
Mitochondria
Lysosomes
What is the structure and function of the mitochondria?
Double membrane: controls entry and exit of material
Cristae (extensions of inner membrane): provide large surface area for enzyme attachment
Matrix: contains proteins, lipids, ribosomes, DNA that allow mitochondria to control production of proteins
What is the structure and function of the chloroplast?
Chloroplast envelope: controls entry and exit of substances
Grana: stacks of thylakoids which contain the chlorophyll
Granal membranes provide large surface area
Stroma: contain starch grains
Grana & stroma: both for photosynthesis
What is the structure and function of the chloroplast?
Chloroplast envelope: controls entry and exit of substances
Grana: stacks of thylakoids which contain the chlorophyll
Stroma: contain starch grains
Grana & stroma: both for photosynthesis