Section 15 Flashcards
What is impedance and direct current in coulter principle
Cells displace small amount of electrolyte when traveling which correlates to cell size
Impedance eliminates cell recirculation
How are laser lights used in cell counts
Scattered light measures cell surface granularity
What do radio waves measure (conductivity)
Measures internal cell structures
What is hydrodynamic focusing
Laminar flow ensures single cell lines
What is sweepflow
Allows single cell passage and measures cells exiting at odd angles
What is fluorescent flow cytometry
Measurement and analysis as single cells pass
How are thresholds and channels used for counting
Threshold: size limit
Channel: area between thresholds
How are histograms used in cell analysis
Measures size of cells and plots amount counted of those sizes to determine cell concentrations
What are scattergrams/plots
Each dot represents cell plotted on a 3D charts of conductivity vs scatter vs volume
What does volume in VCS measure
Measures total cell volume with direct current impedance
What does light scatter (s) measure in VCS
Cell shape/ surface with lasers
what does sysmex heme analyzers measure
- RNA/DNA info
- internal structure
- cell size
