Section 13 Flashcards
What are the WBC reference ranges
Male & Female: 4.0-11*10^3/mm^3
What are the male and female PLT reference ranges
Male & female: 150-400*10^3/mm^3
What is the volume of 1 primary square in a neubauer hemocytometer
0.1 mm^3
How many squares are counted for manual white cell count
4 primary squares (corners)
What is the volume of one secondary square
0.004mm^3
How many secondary squares are in one primary square
25
How many squares and what kind are counted for RBC manual counts
5 secondary squares
What is the volume of one tertiary square
0.00025 mm^3
How many of what type of square is counted for PLT manual count
10 secondary squares
What dilutions are used for WBC, RBC and PLT neubauer hemocytometer
WBC: 1:20
RBC: 1:200
PLT: 1:100
What equation is used to determine cell volume in a blood sample using neubauer hemocytometer
WBC or PLT 10^3/mm^3 =
(Avg#cells counteddilution factor*10)/(# of primary squares counted)
Equation to correct WBC for nucleated red cells
=(100*uncorrected WBC)/(100+nRBC)