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Microbiology (bacteria) > Salmonella + Shigella > Flashcards

Salmonella + Shigella Flashcards

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Biology 101 flashcards
1
Q

Does Salmonella ferment lactose?

A

no

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2
Q

How can you identify a lactose negative bacteria

A

MacConkey or EMG agar

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3
Q

Three important characteristics of ALL enterbacteriaceae

A

1) ferment glucose
2) oxidase negative
3) reduce nitrate

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4
Q

Does Salmonella produce H2S?

A

yes

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5
Q

Is Salmonella motile?

A

yes

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6
Q

Is Salmonella urease negative or positive?

A

negative

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7
Q

Is Salmonella indole negative or positive?

A

negative

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8
Q

What is the most common species of shigella in the developing world?

A

dysenteriae

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9
Q

18% of shigella in the US are of this species:

A

flexneri

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10
Q

most common shigella species in the US

A

sonnei

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11
Q

a common shigella species on the indian subcontinent

A

boydii

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12
Q

how is shigella spread?

A

food, fingers, feces, flies

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13
Q

is there bacteremia in shigella?

A

rare

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14
Q

symptoms of shigella

A
  • fever
  • diarrhea
  • bloody diarrhea with mucus
  • abdominal cramps
  • hemolytic uremic syndrome
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15
Q

what is the infectious dose of shigella

A

10-100 organisms

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16
Q

shigella incubation period

A

1-4 days

17
Q

explain the pathogenesis of shigella

A
  • invade intestine in terminal ileum and colon
  • uptake by macrophages (T3SS dependent)
  • escape and cell to cell spread (T3SS indep)
  • apoptosis of macrophage
  • IL-1 and TNF from monocytes lead to fever
  • shiga toxin causes intestinal ulcerations
  • shiga toxin subunit A interferes with 60S ribosomal RNA inhibiting protein synthesis
18
Q

can shigella be diagnosed based on clinical symptoms?

A

no

19
Q

does shigella produce gas with glucose fermentation?

A

no

20
Q

does shigella ferment lactose?

A

no, like all other enterobacteriaceae

21
Q

does shigella produce H2S?

A

no

22
Q

is shigella motile?

A

no

23
Q

shigella negative or positive for indole? urease?

A

negative for both

24
Q

what antigens does shigella possess?

A

O only.

25
Q

what antigens does salmonella possess?

A

O and H.

Microbiology (bacteria) (16 decks)

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