S1 Flashcards
What is Schedule 1?
List of methods for humanely killing animals
It is a legal requirement after conducting a schedule 1 procedure to…
Confirm death of that animal
Give some reasons why it may be appropriate to euthanize an experimental animal
- Scientific endpoint (EOP)
- continuing adverse effects (Humane endpoint)
- Levels of pain/distress likely to exceed severity limit
- collection of tissue for scientific purpose
- Animals no longer suitable for breeding
- Surplus animals : unwanted stock/ unsuitable characteristics
How is killing of animals regulated by ASPA?
ASPA15A permits relevant PROTECTED ANIMALS to be KILLED INTENTIONALLY only by a COMPETENT PERSON using a METHOD DEFINED AS APPROPRIATE
Who can kill a protected animal?
- registered competent person in an establishment licence holder register
- adequately educated
- supervision required until competence is demonstrated
Where can protected animals be killed?
In a designated area in the licenced establishment (e.g. Sc1 room)
Is a PIL required for schedule 1 killing?
No
- but must be competent and on the register
What is required for use of non-schedule 1 methods of killing?
Requires PIL and PPL licence authority
What 6 methods can be used to ensure animals are dead?
- Permanent cessation of circulation
- Rigor mortis onset
- Cervical dislocation
- Destruction of the brain
- Exsanguination
- MEchanical disruption
What basic 3 mechanisms do euthanasia techniques cause death by?
- direct depression of neuronal activity necessary for life function
- hypoxia
- physical disruption of brain activity
When can the use of sedation/anaesthesia be used when carrying out S1?
As long as its use does not cause greater distress than performing the technique without a sedative or anaesthetic
What 5 methods of killing are appropriate for animals which ARE NOT of foetal, larval or embryonic forms?
- overdose of anaesthetic
- Exposure to Co2 in rising concentration
- Dislocation of the neck
- Concussion of the brain
- Slaugher (ungulates)
What 4 methods of killing are appropriate for animals which are of foetal, larval or embryonic forms?
- overdose of anaesthetic
- refridgeration/disruption of membranes/maceration
- cooling of foetuses
- Decapitation
What method is appropriate for both killing of adult and embryonic forms?
- overdose of anaesthetic
Is overdose of anaesthetic appropriate for all animals?
Yes
What route can overdose of anaesthetic be applied?
IV or IP depending on animal
- volatiles/gases would take too long to cause death
What animals are exposure to rising CO2 apporpriate for?
Rodents, rabbits and birds up to 1.5kg
How quickly should the chamber be filled up with co2?
20% per minute
What animals is dislocation of the neck appropriate for?
- rodents up to 500g
- rabbits up to 1kg
- birds up to 1kg
What animals must be sedaed before cervical dislocation?
rodents and rabbits which are more than 150g
- birds bigger than 250g
What animals is concussion of the brain suitable for?
- rodents and rabbits up to 1kg
- birds up to 250g
amphibians and reptiles up to 1kg - fished
Give some recognised methods of slaughter
- destruction of the brain by free bullet
- captive bolt, ekectrical stunning followed by destruction of the brain ot exsanguination
What protected animals must humane killing/sc 1 be used for?
any mammal, bird or reptile from 2/3 of the way through gestation, or incubation period, or any fish/amphibian from stage where it capable of independent feeding
When is decapitation appropriate for embryonic mammals and birds?
When they are <50g
- small foetuses and avian embryos - sharp pair of sharp scissors
what dose of pentobarbitone may be appropriate for euthanasia?
100-150mg/kg
Hw long must animals remain in 100% co2 for to ensure that they are dead?
10 mins
