S1

Question 1

What is Schedule 1?

Answer 1

List of methods for humanely killing animals

Question 2

It is a legal requirement after conducting a schedule 1 procedure to…

Answer 2

Confirm death of that animal

Question 3

Give some reasons why it may be appropriate to euthanize an experimental animal

Answer 3

• Scientific endpoint (EOP)
• continuing adverse effects (Humane endpoint)
• Levels of pain/distress likely to exceed severity limit
• collection of tissue for scientific purpose
• Animals no longer suitable for breeding
• Surplus animals : unwanted stock/ unsuitable characteristics

Question 4

How is killing of animals regulated by ASPA?

Answer 4

ASPA15A permits relevant PROTECTED ANIMALS to be KILLED INTENTIONALLY only by a COMPETENT PERSON using a METHOD DEFINED AS APPROPRIATE

Question 5

Who can kill a protected animal?

Answer 5

• registered competent person in an establishment licence holder register
• adequately educated
• supervision required until competence is demonstrated

Question 6

Where can protected animals be killed?

Answer 6

In a designated area in the licenced establishment (e.g. Sc1 room)

Question 7

Is a PIL required for schedule 1 killing?

Answer 7

No

- but must be competent and on the register

Question 8

What is required for use of non-schedule 1 methods of killing?

Answer 8

Requires PIL and PPL licence authority

Question 9

What 6 methods can be used to ensure animals are dead?

Answer 9

• Permanent cessation of circulation
• Rigor mortis onset
• Cervical dislocation
• Destruction of the brain
• Exsanguination
• MEchanical disruption

Question 10

What basic 3 mechanisms do euthanasia techniques cause death by?

Answer 10

• direct depression of neuronal activity necessary for life function
• hypoxia
• physical disruption of brain activity

Question 11

When can the use of sedation/anaesthesia be used when carrying out S1?

Answer 11

As long as its use does not cause greater distress than performing the technique without a sedative or anaesthetic

Question 12

What 5 methods of killing are appropriate for animals which ARE NOT of foetal, larval or embryonic forms?

Answer 12

• overdose of anaesthetic
• Exposure to Co2 in rising concentration
• Dislocation of the neck
• Concussion of the brain
• Slaugher (ungulates)

Question 13

What 4 methods of killing are appropriate for animals which are of foetal, larval or embryonic forms?

Answer 13

• overdose of anaesthetic
• refridgeration/disruption of membranes/maceration
• cooling of foetuses
• Decapitation

Question 14

What method is appropriate for both killing of adult and embryonic forms?

Answer 14

• overdose of anaesthetic

Question 15

Is overdose of anaesthetic appropriate for all animals?

Answer 15

Yes

Question 16

What route can overdose of anaesthetic be applied?

Answer 16

IV or IP depending on animal

- volatiles/gases would take too long to cause death

Question 17

What animals are exposure to rising CO2 apporpriate for?

Answer 17

Rodents, rabbits and birds up to 1.5kg

Question 18

How quickly should the chamber be filled up with co2?

Answer 18

20% per minute

Question 19

What animals is dislocation of the neck appropriate for?

Answer 19

• rodents up to 500g
• rabbits up to 1kg
• birds up to 1kg

Question 20

What animals must be sedaed before cervical dislocation?

Answer 20

rodents and rabbits which are more than 150g

- birds bigger than 250g

Question 21

What animals is concussion of the brain suitable for?

Answer 21

• rodents and rabbits up to 1kg
• birds up to 250g
  amphibians and reptiles up to 1kg
• fished

Question 22

Give some recognised methods of slaughter

Answer 22

• destruction of the brain by free bullet

- captive bolt, ekectrical stunning followed by destruction of the brain ot exsanguination

Question 23

What protected animals must humane killing/sc 1 be used for?

Answer 23

any mammal, bird or reptile from 2/3 of the way through gestation, or incubation period, or any fish/amphibian from stage where it capable of independent feeding

Question 24

When is decapitation appropriate for embryonic mammals and birds?

Answer 24

When they are <50g

- small foetuses and avian embryos - sharp pair of sharp scissors

Question 25

what dose of pentobarbitone may be appropriate for euthanasia?

Answer 25

100-150mg/kg

Question 26

Hw long must animals remain in 100% co2 for to ensure that they are dead?

Answer 26

10 mins