RP9- EFFECT OF TEMP ON RESPIRATION IN YEAST

Question 1

Why do you leave the tubes in the water bath for 5 mins

Answer 1

so yeast and glucose mixture can reach equilibrium

Question 2

what would be a control experiment and why

Answer 2

test tube with just methylene blue
just glucose
just glucose and BOILED yeast mixture

to show that glucose doesn’t cause meth blue to go colourless/colour change as due to respiration

Question 3

why would shaking the tubes cause meth blue to go blue again

Answer 3

mixed with O2 in the air
O2 oxidises meth blue

Question 4

pressure exp:
why is it important to leave the apparatus for 1hr after yeast culture reached a constant temp

Answer 4

so that O2 is respired/absorbed

Question 5

why is a log scale used to record the number of cells

Answer 5

large range in n.o of cells

Question 6

what measurements should be taken to calculate the rate of aerobic respiration in mm3/g-1/h-1

Answer 6

distance and time moved by liquid
diameter of tube
mass of woodlouse