RP6-ASEPTIC TECHNIQUES

Question 1

why do you boil the agar

Answer 1

to remove any unwanted bacteria so no contamination

Question 2

why do you transfer same volume of liquid culture onto each agar plate

Answer 2

so same no of bacteria transferred
to allow comparison

Question 3

describe some aseptic techniques when transferring broth culture to agar plate

Answer 3

flame neck of bottle to maintain pure culture of bacteria

lift agar lid at little as possible when transferring to prevent any unwanted bacteria entering from air/breath

wear gloves to prevent contamination of bacteria from hands