RP1- EFFECT OF VARIABLE ON ENZYME-CONTROLLED REACTION

Question 1

why are buffer solutions used

Answer 1

maintains PH of solution

Question 2

you left test tubes in water baths for 10 mins before adding enzyme explain why

Answer 2

to reach the temperature at which the reaction will occur

Question 3

describe what you did to maintain temperatures of water baths so were reliable

Answer 3

use a thermometer to measure temp constantly
if temp drops reheat
keep temp close to required temp

Question 4

why did you set up 3 experiments for each temperature

Answer 4

identify anomalies
repeatability enables calculation of a reliable mean

Question 5

suggest control for this experiment

Answer 5

boiled trypsin enzyme = denatured so no effect

Question 6

student concluded that 40 degrees was optimum temperature , evaluate conclusion (give a limitation)

Answer 6

conclusion not valid as 40 degrees is only temperature investigated

Question 7

describe how you can change method to make it more reliable

Answer 7

carry out 5 repeats
and calculate mean/average

Question 8

Maltose concs
describe how you would produce calibration curve and used it to obtain results in graph

Answer 8

use maltose solutions of known concentrations

use a coloromiter to measure colour volume of each solution and plot calibration curve

find conc of sample from calibration curve