RP2- ROOOT TIP SQAUSH

Question 1

describe how temporary mounts of plant tissue are made

Answer 1

slice a thin slice
put slide in water
add cover slip

Question 2

describe how you would use the mounts of leaves to determine mean n.o of chloroplasts in cells of leaf

Answer 2

select large no of cells at random
count n.o of chloroplasts in field of view
divide no of chloroplasts by n.o of cells

Question 3

why is it important that thin sections of tissue should be used

Answer 3

so light can pass through
a single layer of cells can be viewed

Question 4

why do you ensure you dont move the coverslip

Answer 4

to prevent cells overlapping each other

Question 5

explain why a root tip was used

Answer 5

where mitosis occurs

Question 6

explain why a stain was used

Answer 6

distinguishes chromosomes making them visible
shows which cells undergoing mitosis

Question 7

why was root tip firmly squashed

Answer 7

so thin layer to let light through