RP2 - Calculating Mitotic Index using Plant Cells Flashcards

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1
Q

Where in plants can cells undergoing mitosis be found?

A

Meristem tissue at shoot and root tips.

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2
Q

What is the mitotic index?

A

The ratio of cells undergoing mitosis to the total number of cells in a sample.

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3
Q

Outline the procedure to prepare a root tip slide.

A
  1. Warm 1M HCl to 60°C in a water bath.
  2. Cut a root tip using a scalpel and add to the HCl. 3. Leave for 5 minutes.
  3. Remove from HCl and wash with distilled water.
  4. Cut the tip of the root tip sample and place on a slide.
  5. Add a few drops of stain to make chromosomes visible.
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4
Q

State the formula for the mitotic index.

A

Mitotic index = Number of cells with visible chromosomes / Number of cells in sample

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5
Q

State the hazards and precautions for reagents used in this procedure.

A

HCl - corrosive, avoid skin contact, wear eye protection
Toluidine Blue O stain - irritant, avoid contact with
skin, wear eye protection
Scalpel - cut away from fingers

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