RP 1 - Using a Light Microscope

Question 1

How do you Prepare a Microscope Slide? (4 Steps)

Answer 1

1 ) Add a drop of Water to the Microscope Slide.
2 ) Place a Thin Layer of Tissue on the Slide.
3 ) Stain the Tissue with a couple drops of Iodine Solution.
4 ) Place the Coverslip on top.

Question 2

How do you Observe a Microscope Slide? (4 Steps)

Answer 2

1 ) Place the Slide on the Stage and use the Lowest Power Objective Lens.
2 ) Turn the Coarse Focussing Dial to bring the Image into Focus.
3 ) Increase the Power of the Objective Lens to Increase Magnification.
4 ) Turn the Fine Focussing Dial to bring the Image into clearer Focus.

Question 3

Why should you Stain Sample Cells?

Answer 3

So that you can clearly see Sub-Cellular Structures.

Question 4

What could you use to Stain the Sample Cells?

Answer 4

Iodine Solution

Question 5

What is the Risk of Iodine Solution?

Answer 5

May cause an Allergic Reaction or Skin Rash.

Question 6

How can you Minimise the Risk of Iodine Solution?

Answer 6

• Wear Gloves & Goggles
• Clean up Spills Immediately

Question 7

Which Lens do you first use when Viewing a Cell under a Microscope?

Answer 7

The Lowest Power Objective Lens

Question 8

How do you Increase the Magnification of the Image?

Answer 8

Increase the Power of the Objective Lens.

Question 9

How do you bring the Image into a clearer Focus?

Answer 9

Turn the Fine Focussing Dial to Adjust the Height of the Stage.

Question 10

If the Image through the Eyepiece Lens of a Microscope is Fuzzy what should you do?

Answer 10

Turn the Fine Focussing Dial to bring the Image into Focus.