1.3 - Microscopy Flashcards
Where do you mount a Microscope Slide?
The Stage
How do Light Microscopes work?
1 ) Light from the room hits the light source (mirror).
2 ) Light is reflected upwards through the object.
3 ) Light passes through one of the objective lenses.
4 ) Light passes through the eyepiece lens, into our eyes.
What is Magnification?
How many times larger the image is than the object.
What is the equation for Magnification?
Magnification = Image Size / Object Size
What is the definition of Resolution?
The shortest distance between two points on an object that can still be distinguished as two separate entities.
What are some advantages of Light Microscopes?
- Small & Easy to Use
- Relatively Cheap
- Can view in Colour
- Can view Living Specimens
What are some disadvantages of Light Microscopes?
- Low Magnification
- Resolution is limited to 0.2μm
- Not good enough to study sub-cellular structures
What do Light Microscopes rely on?
Light (Wavelength of 0.2μm)
What are some advantages of Electron Microscopes?
- Higher Magnification
- Maximum resolution of 0.1nm
- Can be used to study sub-cellular structures
What are some disadvantages of Electron Microscopes?
- Very Expensive
- Hard to Use
- Cannot view in Colour
- Cannot view Living Specimens
What do Electron Microscopes rely on?
Beams of Electrons (Wavelength of 0.1nm)
How do you convert from mm to μm?
Multiply by x1,000
How do you convert from μm to nm?
Multiply by x1,000
How do you convert from μm to mm?
Divide by /1,000
How do you convert from nm to μm?
Divide by /1,000
