Rotavirus

Question 1

Rotavirus Baltimore

Answer 1

dsRNA

Question 2

Rotavirus Virion

Answer 2

Inner capsid, imtermediate capsin, outer capsid

6 structural VP- VP1,3,2,6,7,4

Question 3

rotavirus genome

Answer 3

non structural proteins in genome rep and antiviral defense
NSP 1-6
replicates in mature non-dividing enterocytes in small intestine.

Question 4

Rotavirus attachment and entry

Answer 4

Attachment mediated by VP4

Receptors include integrins, sailic acid, tight junction proteins

Question 5

Rotavirus disease

Answer 5

Is shed in stool and transmits through fecal-oral route.
Infects enterocytes, induces diarrhea by activation of ENS
Weakens tight junctions between cells and allows leakage to occur

Question 6

Rotavirus treatment

Answer 6

Treatment occurs by replacing fluids and electrolytes.

Question 7

Rotavirus Pathogenesis

Answer 7

NSP4 induces CFTR diarrhea and destroy enterocytes

Question 8

Reovirus Viral benefit **

Answer 8

host cell: DSRNA detected- PKR activated-phosphorylates EIF2a- shuts down translation- remains bound and virus cant translate proteins
Virus: Permanently turn on RAS pathway(mutated) so PKR is turned off and cannot phosphorylate and translation can continue.

Question 9

Oncolytic reoviruses treatment for cancer

Answer 9

only targets cancer cells b/c pkr is turned on perm in those cells and lyse them
only infects tumor cells b/c enterocytes