RNA Transcription

Question 1

RNA polymerase in eukaryotes primarily associated with the synthesis of rRNA

Answer 1

RNA pol 1 (MRT 213)

Question 2

RNA polymerase in eukaryotes associated with the synthesis of mRNA, snRNA and other noncoding RNA

Answer 2

RNA pol 2 (MRT 213)

Question 3

RNA polymerase in eukaryotes associated with the synthesis of tRNA and rRNA

Answer 3

RNA pol 3 (MRT 213)

Question 4

Enumerate the steps in Transcription

Answer 4

1: RNA polymerase and cofactors bind to DNA
2: Sigma subunit recognizes promoter sequence
3: Promoter clearance
4: Elongation 5’-3’
5: Termination: at terminator sequence or with Rho-Protein

Question 5

This sequence relevant to transcription is A-T rich to allow unwinding

Answer 5

Promoter sequence

Question 6

This sequence relevant to transcription is composed of a series of bases that form a stable semi-loop, and a series of uracil

Answer 6

Terminator sequence

Question 7

True or False: RNA’s proofreading capacity is due to the 3’-5’ proofreading of RNA pol 3

Answer 7

False. RNA has NO proofreading capacity

Question 8

RNA that contains both introns and exons

Answer 8

pre-mRNA

Question 9

These are enzymes responsible for the removal of introns in mRNA

Answer 9

spliceosomes

Question 10

True or False: mRNA may splice out introns without spliceosomes

Answer 10

TRUE! :)

Question 11

This mRNA activity in relation to splicing explains why there is an expanding versatility of genomic sequences, combinatorial control and mixing and matching of exons

Answer 11

Alternative splicing

Question 12

What are the two post-transcriptional modifications in mRNA that allows it to avoid degradation in the cytosol

Answer 12

5’ Methyl Cap and 3’ poly-A tail

Question 13

RNA component of ribosomes

Answer 13

rRNA

Question 14

RNA attached to an amino acid

Answer 14

tRNA

Question 15

RNA involved in RNA interference in animals

Answer 15

miRNA (siRNA in plants)

Question 16

RNA involved in controlling/’silencing’ transposable elements and directing methylation

Answer 16

piRNA (piwiRNA)

Question 17

non-coding RNA that directs epigenetic machinery to particular sites

Answer 17

lncRNA