Protein Synthesis

Question 1

Start Codon

Answer 1

AUG- Methionine

Question 2

Stop codons

Answer 2

UAG UGA UAA

Question 3

Initiation factors involved in the Dissociation complex. What is their role?

Answer 3

eIF-3 and eIF-1A. ‘Stilts’ Delay the reassociation of small and big subunits

Question 4

Initiation factor involved in the 43S Preinitiation complex. What is its role?

Answer 4

eIF-2. binds to GTP and Met-tRNA and then to 40S

Question 5

Initiation factor involved in the 48S Initiation complex. What is it’s subgroups and role/s?

Answer 5

eIF-4F.
eIF-4G- Scaffolding
eIF-4E- recognize and bind methyl cap
eIF-4A and 4B- melts the secondary structure of the cap

Question 6

This sequence contributes to the determination of the start codon

Answer 6

Kozak consensus sequence

Question 7

Initiation factor involved in the 80S initiation complex. What is its role?

Answer 7

eIF-5. hydrolyzes GTP bound to eIF-2, removes all other eIFs

Question 8

What are the subphases in the initiation phase of Protein translation

Answer 8

Dissociation complex, 43s Pre-initiation, 48S initiation, 80S initiation

Question 9

Elongation factor associated with A-site attachment. What is its role?

Answer 9

eEF-1alpha. Binds with entering aa-tRNA and GTP, allowing its attachment to the A-site

Question 10

What enzyme is involved in peptide bond formation?

Answer 10

peptidyl transferase

Question 11

Elongation factor associated with translocation. What is its role?

Answer 11

eEF-2. Binds to GTP. Uses energy to push tRNA in A site to P site

Question 12

Energy requirement in elongation (in phosphate bonds)?

Answer 12

4 high energy phosphate bonds

Question 13

Releasing factor associated with the recognition of UAA and UAG

Answer 13

eRF1

Question 14

Releasing factor associated with the recognition of UAA and UGA

Answer 14

eRF2

Question 15

Releasing factor that binds with GTP

Answer 15

eRF3