RNA processing

Question 1

5’ splice site

Answer 1

GU-AAGU (or something similar)-GU is important

Question 2

Branch site

Answer 2

Catalytic A close to 3’ splice site

Question 3

3’ splice site

Answer 3

AG requirement then conserved run of U or C

Question 4

splicing mechanism

Answer 4

3’ AG, phosphate bond to catalytic A 2’OH, 3’AG to the phosphate is cleaved making into 3’OH, phosphate bond to phosphate by 5’ G-slide 7
2’OH is a nucleophile that breaks phosphate bond
The bonds form/break are called transesterification
DRAW

Question 5

SNURPS+parts of it

Answer 5

wraps sequence in a knot

U1/U2-bring 5’ and 3’ splice site in 3D proximity

Question 6

Alternative spliciing

Answer 6

Idea of being able to keep some exons and not others to make differing proteins
Result in many different related proteins form a single gene

Question 7

Beta thallasemia

Answer 7

Mutation in intron, splicing occurs more upstream-more amino acids in gene