RNA Flashcards
RNA function
Decode DNA into proteins
Ribosomes
rRNA + proteins
Translate mRNA codons into particular amino acids
tRNAs
T of F: RNA functions as genome (self-replicating in RNA viruses)
True
Ribozyme
RNA functioning as enzyme
Function: Cleavage and rejoining of polynucleotide chains
Ribozyme
Function: RNA splicing
Ribozyme
snRNA
Small, nuclear RNA
Function: participate in RNA processing and DNA replication
snRNAs
How are RNAs classified?
According to size
Sediment coefficient
S, 10^-13 s
Zonal centrifugation
Separates particles of different sizes
Which RNAs do not separate as discrete fraction?
mRNAs
Which RNA is most abundant ?
rRNA
rRNAs fold into what degree of structure ?
Secondary
RNAs required for translation
tRNAs
RNAs that fit the cloverleaf model
tRNAs
Specific tRNAs
Accept only 1 of the 20 AAs
Isoaccepting tRNAs
Different tRNAs that bind the same AA
Acceptor stem (tRNA)
+ Nucleotide base pairs that extend to unpaired CCA at 3’ end
+ Site of amino acid conjugation
TSiC loop
Contains pseudouridine
Anticodon
3 nts that interact with mRNA
D loop
Contains dihydrouridine residue
T or F: In mitochondria animals do contain full set of tRNAs
True
T or F: In mitochondria plants do contain a full set of tRNAs
False
Aminoacyl transferase
May participate in tRNA import
T or F: In plastids plants do contain a full set of tRNAs
True
T or F: Plant cytoplasmic tRNAs are similar to other eukaryotes
True
Number of mRNA species =
Number of different proteins
Function: Pre-mRNA splicing
snRNAs
Function: Histone pre-mRNA processing, poly(A) addition
snRNAs
Location of snRNAs
Nucleoplasm
snoRNAs
Small nucleolar RNAs
Location of snoRNAs
Nucleolus
Function: Pre-rRNA processing
snoRNAs
