RNA

Question 1

What is the difference between the Quick-RNA and Direct-zol kits? Do they perform the same?

Answer 1

The Quick-RNA and Direct-zol kits produce similar yield and quality of RNA. The main difference is that the Direct-zol kit uses TRI Reagent or TRIzol as a lysis buffer where the Quick-RNA uses a guanidine based buffer. For customers that prefer to use TRIzol, the Direct-zol kit is best. Otherwise, the Quick-RNA system is recommended

Question 2

What is the difference between the Quick-RNA Miniprep and Quick-RNA Miniprep Plus kits?

Answer 2

Use the Quick-RNA Miniprep for cells and soft tissues. The “Plus” kit accommodates all sample types (cells, tissue, blood) and comes with DNA/RNA Shield (sample collection, transport, storage at ambient temperature)

Question 3

Will the Direct-zol/Quick-RNA kit isolate small RNAs?

Answer 3

Yes, the kit recovers small and microRNA down to 17nt

Question 4

Can samples be stored in RNA Lysis Buffer?

Answer 4

Yes, samples in RNA Lysis Buffer are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.

Question 5

Is it possible to extract proteins with the Quick-RNA kits?

Answer 5

Yes, proteins can be acetone precipitated from the column flowthrough. Please see the Protein Purification appendix in the protocol.

Question 6

What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?

Answer 6

Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.

Question 7

I ran out of RNA Wash Buffer. Can I use something else?

Answer 7

Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.