Rh Typing

Question 1

It detects the presence of D antigen on patient’s RBC

Answer 1

Routine Rh typing

Question 2

In slide method at what temp is in the Rh viewbox and read at what temp?

Answer 2

45-50oC and read at 37oC

Question 3

In tube method, what are the steps until a positive result show

Answer 3

1) add anti-D and centrifuge
2) incubate at 37oC and spin
3) Add AHG
4) Add check cells

Question 4

If Rh typing shows no agglutination and indicates Rh (-) should be confirmed by

Answer 4

Weak D testing

Question 5

T/F: Rh typing has a reverse typing

Answer 5

False: Rh antibodies are not naturally occurring

Question 6

Type of Anti-D antisera: IgM, low protein, lengthy incubation, not used for weak D

Answer 6

Saline Anti-D

Question 7

Type of Anti-D antisera: IgG, material used was human plasma with high titer D-specific antibody, cause RBCs to be in close proximity allowing crosslink and agglutination

Answer 7

High Protein Anti-D

Question 8

Type of Anti-D antisera: IgG-modified, low protein, used for slide and tube methods

Answer 8

Chemically modified Anti-D

Question 9

Weak D testing principle follows what procedure

Answer 9

IAT

Question 10

When is Weak D testing performed?

Answer 10

When Rh typing shows negative results

Question 11

Weak D (+) patient are considered

Answer 11

Rh (-)

Question 12

Weak D (+) donor is considered

Answer 12

Rh (+)