Review Questions Exam # 1 Flashcards
What are the key characteristics of “Parasitism”?
- Form of symbiosis.
- Parasites must depend on the host for survival
- Have adverse effects on host.
- Has greater fecundity than host.
- Is usually smaller than the host.
- Doesn’t want to kill its host.
- Are over dispersed among the host population (macro-parasites).
What is the difference between direct and indirect lifecycle?
Direct Lifecycle: Larvae/ egg of parasite have free living stage . No intermediate hosts.
Indirect lifecycle: Sexual reproduction occurs in the definitive host, but there are intermediate hosts that could transport the parasite/ allow for larval development and/or paratenic hosts that are just for transport. There are no free living stages.
What are the shed stage and infective stage of a parasite?
• Shed stage of a parasite (L1 -L2)
◦ Stage that eggs can be found. Diagnostic phase.
• Infective stage is L3 (L1 in urine in some species) ◦ Infect definitive host
What is the biological role of Intermediate hosts and paratenic hosts in a parasite’s lifecycle? Do parasites need paratenic hosts to complete their lifecycle?
Not all parasites need paratenic hosts to complete their lifecycles only some. Intermediate hosts allow for larval development and transport of parasites while paratenic hosts do not allow for larval development.
A dog is considered as the definitive host of Toxocara canis, why?
Main host. Supports sexual reproduction of the parasite. Parasite reaches sexual maturity within this host
What are two key differences between a microparasite and a macroparasite?
Microparasite
• Tiny, unicellular, microscopic
• Multiply within hosts
• Short lifecycle
Macroparasite
• Large, multicellular, adult microscopic • Reproduce in definitive hosts
• Generally longer lifecycles
What is a facultative parasite?
Both parasitic and non parasitic life cycle. Opportunistic parasite- can complete its life cycle without hosts.
What is the name given to a parasite which doesn’t have a free-living stage?
Obligatory parasite
What are spurious parasites? What would you call a pollen particle that looks like an egg?
Spurious parasite: Is a parasite found in an unusual host. You would call a pollen particle that looks like an egg an pseudoparasite.
What is the general body plan of Nematodes, cestodes, trematodes, arachnids, insects and
protozoa? Overall morphology (E.g. round, flat, segmented…., macroscopic vs microscopic)
Nematodes: Round (worms) non segmented, cylindrical with tapered ends, elongated, , macroscopic, complete digestive system. Multicellular
Cestodes: Dorsally flattened, with segments, macroscopic, multicellular, head with suckers, may contain hooks.
Trematodes: Oval shaped, flat, macroscopic, multicellular, leaf like body, Two suckers, oral and ventral
Arachnids: 8 legs, 2 body segments) macroscopic, multicellular
Insects: 6 legs, 3 body segments), macroscopic, multicellular
Protazoa : Unicellular, microscopic
List the main transmission routes of parasites?
- Fecal- oral
- Skin (per-cutaneous)
- Vector transmission/ inoculation
- Direct contact
• Sexual transmission
• vertical transmission
• Iatrogenic
• Transmammary/lactogenic
• Trans placental/ prenatal
What are the common names of nematodes, cestodes and trematodes?
Nematode- roundworm Cestode- Tapeworm Trematode: Flukes
What is the outer covering of a nematode, cestode, trematode?
Nematode: Chitinous Cuticle
Cestode: Tegument Trematode: Tegument
Do nematodes have sexual dimorphism? What about cestodes, and trematodes?
Nematodes have sexual dimorphism. Males are smaller than females, Copulatory bursa in males.
Cestodes: They are hermaphrodites They have self fertilization
Trematodes: Also hermaphrodites other than schistostomes
Which larval stage is generally the infective stage in the case of nematodes? Which stages of larvae are inside the definitive host?
Generally L3 is the infective stage (L1 can be in some parasites). The stages of larvae that are within the host are L3-L5
What is the general term given to inhibited larval development in the case of nematodes?
• HYPOBIOSIS in many nematode species (e.g Trichostrongyles)
◦ Inhibited larval development to avoid adverse conditions for development and
maturation – adaptation to host and environmental conditions.
What is the key difference between cyclophylidean and Pseudophylidian cestode? Just the head morphology?
The key difference between cyclophylidean and pseudophylidian is that cyclophylidean cestodes have non operculated eggs and have a sucker attachment at the head. In pseudophylidia, they have operculated eggs and a bothria
What are proglottids? What is a tegument?
Proglottids are the body segments of tape worms. Each proglottid has both male and female reproductive system present.
Tegument- is the skin like covering the parasite feeds through/ absorbs nutrients through. (in terms of cestodes).
What is unique about a Taeniid-type egg?
All Taenia species (Taenia spp., Echinococcus spp.) have same egg morphology- commonly called taeniid eggs.
◦ The Eggs have an embryo with 6 hooks (hexacanth larvae) and thick straight egg shell.
Do trematodes have a direct lifecycle? What are the most common intermediate hosts?
Trematodes always have an indirect life cycle. Must have 2-3 hosts. Intermediate host is always a snail/ slug (aquatic/ terrestrial)
What is a thorny-headed worm?
Acanthocephalans
- Small phylum of highly specialized parasites of the vertebrate digestive tract
- Retractable, spiny attachment organ or proboscis
- No digestive tract, nutrient absorbed through the tegument
- Sexes separate
- Lifecycle indirect – Arthropod intermediate host (beetles)
Examples
◦ Macracanthorhynchus – Swine,
◦ Oncicola – Canine
Not a very common group of parasites**
What is the infective stage of most trematodes?
Metacercaria
General morphological difference (gross body) between arachnids and insects? No. of legs, segments?
Arachnids: 2 distinct body parts (Cephalothorax and abdomen) 8 legs as adults, sometimes 6 at nymph stage. No wings/ antennae
Insects: Three distinct body parts ( Head, Thorax, Abdomen, 6 legs (sometimes: 2 antennae, 2 pairs of wings)
What are the two categories of mites and lice? What are the key morphological differences between the categories of mites and lice? Or, what main feature would you look for if you had to identify these groups of mites and ticks?
Mites:
• Burrowing : under surface, small legs, round body, smaller
• Surface: on surface, longer legs, oval body, larger
Lice:
• Chewing Lice: Wider head (wider than thorax) Stout mandibles
• Sucking Louse: Head narrower than thorax. Piercing mouthparts
Do mites survive in the environment for a long time? Can a dog give mite to another dog? How?
Limited environmental survival, some species only. Yes they can via contact and grooming instruments. (Direct and indirect)
Same question as above for lice, ticks, fleas, and bugs.
Lice: Entire lifecycle is 20-40 days on host and can live 2-3 weeks off host but usually less than a week. Direct or indirect contact can transmit ( so direct contact and use of shared grooming tools ect.)
Fleas: Life cycle includes environmental time. Can be from sharing bedding, space, toys ect.
Bugs: Adults live for up to 1 year and may survive long period without feeding. Live mostly in environment. No because they do not live on the host, they come out and come into contact with host for blood meal.
Ticks: Life cycle seasonal: one year to multi year. In theory they can, because if a nymph happens to jump off the host and molt and then jump on another dog that could be possible
What is the plate on the back of a hard tick?
Scutum
Point out the key morphological difference between a hard tick and a soft tick? Which kind of tick survives the dry and hot conditions better?
Hard Tick:
• Mouthparts visible from above
• Found in habitats rich in
vertebrates.
• Plate on back called scutum
Soft Tick:
• Mouthparts invisible from above
• Lack Scutum.
• Found in animal burrows,
• Can survive hot and dry
Soft ticks survive dry/ hot conditions better.
conditions
On a three-host and one host ticks, which stages are found in the environment, and on the host?
One host tick: from oviposit to 6 legged larva. Then it completes two molts on the host.
Three host tick: Oviposit -> 6 legged larva, then feeds on host 1 and hops off and molts to 8 legged nymph, then feeds on 2nd host and hops off and molts to adult where it jumps on definitive host and feeds/ mates
How do ticks get on their hosts? Do they fly/Jump?
No jumping/ flying. They find a new host by questing and then grab onto the host when they walk by.
Where do you find bugs most of the time?
Mostly feed at night, during day hide in crevices
Can you differentiate burrowing vs surface mite based on length of legs?
Yes, burrowing mites have short stumpy legs while surface mites have long legs.
Which ectoparasite causes mange?
Mites: Specifically scarcoptes.
What is myiasis? Do the myiasis causing flies bite the host?
• Damage is caused by maggots (larvae) that feed on host tissues • Adults are non-biting
Which arthropod is most host specific and which is least host specific?
Order of decreasing host specificity
1.) Lice 2.) Mites 3.) Fleas 4.)Ticks 5.) Flies
What specialized organs do flagellates and apicomplexans have that help in locomotion and diagnosis?
Flagellates: Flagella Apicomplexan: Gliding locomotion
What is the active motile form of flagellates? What is the environmentally resistant stage?
Trophozoite is the motile form of flagellates.
Cysts are the non motile and environmentally resistant stage.
What are the roles of merogony, gametogony and sporogony in the lifecycle of an apicomplexan parasite?
Merogony: Asexual reproduction ( increase parasitic load within host)
Gametogony Sexual reproduction (Creates fertilized zygotes.
Sporogony: Sporulation of oocyst which become infectious to other dogs.
What is the definitive host in the case of blood Apicomplexa like Babesia?
The tick or arthropod vector is the definitive host. This is because it is where sexual reproduction can occur.
What are the key limitations of fecal smear as a parasitological test?
- Negative result is inconclusive, positive result equally valid.
- Conditions must be ideal i.e fresh sample, refrigerated not frozen,
What is the principle of the fecal flotation method?
Based on differential specific gravity of parasite eggs/cysts, fecal debris and flotation solution. Cysts/ eggs will float to top due to lower specific gravity
Which is the more sensitive test, passive, or active/centrifugal flotation? Why?
Centrifugal fecal floatation is more sensitive. This is because it will recover more eggs/ ova
Which fecal test is preferred for the diagnosis of trematode eggs? Why?
For eggs with higher specific gravity (e.g trematodes and acanthocephalan eggs) that do not float easily or eggs that are easily distorted by flotation solution
Which test is indicated for parasites shedding larvae in the feces?
• Baermann method
Would the Baermann technique be sensitive enough for old, frozen feces? Reason?
Larvae must migrate out of the feces into the water so it must have fresh feces with live larva
Why fecal testing for parasites may not always be reliable?
1.) Chances of false negatives (negative test result despite host having parasite)
• Sporadic shedding
• Prepatent period – Parasites not yet mature to shed eggs/larvae
• Single-sex nematode infections
• Senescent infections
• Old feces (hatched eggs)
2.) Chances of false positives (positive test result but no parasitism)
• Coprophagia/Predation – Spurious parasites
• Pseudoparasites
• Many parasite species shed identical eggs – Diagnostic challenge
What is the advantage of Knott’s test over direct smear?
More blood is being evaluated at one time so it is more sensitive because there is a higher likelihood you will have microfilaria present in a positive patient.
What tests would you choose to diagnose parasites that live on tissue, and don’t have any environmental stage?
- Tissue Digestion
- KOH Digestion
- PCR
- Skin scrape
What are the limitations of immunological tests?
- Measures immune response to exposure – not necessarily active infection
- Cross-reactivity
- Not universally available
What are the common GI nematodes that can infect dogs and cats?
Spirocerca lupi (Esophageal Worms), Physaloptera (Stomach worms)