review questions Flashcards
Which characteristics are suitable for a therapeutic antibody that is used to treat a tumour away from the blood vessel?
- low affinity; to avoid binding to other substances.
- high half-lives
how to determine percentage of live cells in flow cytometry
Total number of cells: 30
Number of stained cells (dead): 16
Number of alive cells: 14
Cell viability: 14/30 * 100% = 47%
With flow cytometry, you can measure several characteristics of cells. What does the forward scatter (FSC) indicate?
cell size
what is SSC value?
it measures sides scatter it show measurement of side of the cells
what method can you use if you want to know whether a new drug affects INF-γ secretion in T cells.
ELISPOT and CBA (cytokines beads assay)
what do you measure with rocket immunoelectrophoresis
mount of antigen in the sample
In rocket electrophoresis (or other precipitation-based methods), when does precipitation occur?
when amount of antigen and antibody are on equivalent
which way to use when want to amplify signal. when A fluorophore-coupled substrate is turned into a highly reactive radical which covalently links to tyrosine residues nearby. Readout same as ELISA.
tyramide signal amplification
which way to use when want to amplify signal. when Two antibodies binding different epitopes are coupled to nucleotide tags. One AB is coupled to a ssDNA tag, while the other is coupled to a dsDNA tag with a partial overhang complimentary to the ssDNA tag on the first AB. No additional connector is required. Readout by qPCR.
proximity extension assay
which way to use when want to amplify signal. when Two antibodies binding different epitopes on the same or different proteins are coupled to specific ssDNA tags. If the tags are close enough to each other, they can be connected via an oligonucleotide thereby creating a double-stranded region. Readout by qPCR.
proximity ligand assay
gu
