PHAGE DISPLAY 2 for large antibody Flashcards
what is Murine monoclonal antibody
antibody from mouse
what is disadvantage of Murine monoclonal antibody
in human
it has side effect and no cure due to HAMA (human anti mouse antibody)
is human antibody same as mouse antibody
no
how is human antibody generated/ produced from
- phase display selection from antibody gene library
- human mouse antibody gene
what is the evolution of antibodies formats
mouse antibody- chimaeric antibody- humanized antibody- human antibody
what are human monoclonal antibody fragment
- scFv ab fragment: consist of VH and VL variants. this developed less than a month
- Fab antibody fragment
- IgG as full antibody: many months
which antibody can Phage Display using antibody library generate
fragment antibody : scFv ot Fab ab fragments
what benefit do we get by having large library of Antibody gene
- more chance of finding what we want
- important: is cheaper and fast
how does Phage Display work?
what is the principle of phage display
genotype (DNA) coupled with phenotype (protein)
what is Expression and display on phage
phenotype: phase displayed antibody fragment
Genotype: phage display vector, DNA encoding antibody fragments
what are two types of phage display victor
- phage victor
- phagemid vector
they both contain protein 3 and 8
what is the benefit of Phage display selection
- isolation of desired antibodies
what is Humira (Adalimumab)
is first human monoclonal antibody approved
must know
Antibody libraries and phage display
Provide human antibodies
Antibody to self antigens
Antibodies to toxic antigens
Provide high affinity
Provide high specificity
Source for human antibody development Humira on the market (anti-TNF-alfa in RA) CAT
