recombinant antibody

Question 1

what is recombinant antibodies

Answer 1

are mono-specific antibodies produced from the recombinant DNA technique

Question 2

what is the need for recombinant antibodies

Answer 2

• rescue of specificity
• appropriate production of cell line
• humanization
• design of effector function
• functionalization of fragment evolved from phage display
• antibodies generated from isolated cells

Question 3

why there is need for recombinant antibodies

Answer 3

• to rescue specificity encoded by unstable hybridoma and cell line
• being able to transform mouse antibody into human antibody through in vivo
• designing antibody for function

Question 4

what is chimeric antibody

Answer 4

is when V domain of mouse antibody is combined by C domain of human antibody

Question 5

what is CDR grafting antibody

Answer 5

is antibody produced when CDR of mouse is combined with human V domain framework region and C domain

Question 6

how does humanization occurs

Answer 6

• by chimerization
  -CDR grafting
• guided selection

Question 7

what is HAMA (human anti mouse antibody) reaction

Answer 7

is when patient reacted to mouse antibodies as if they are foreign substances

Question 8

what is the benefit of humanization

Answer 8

reduce HAMA reaction and immunogenicity

Question 8

what is guided selection

Answer 8

is antibody produced when they select partly human variant in library is combined with one variant of mouse antibody

Question 9

what problem can humanization cause

Answer 9

immunogenicity is completely avoided because CDR is not only molecule involved in antigen binding so other substance outside the CDR can affect positioning of CDR

Question 10

can mouse antibody be transformed into human antibody and how

Answer 10

yes, in vivo

Question 11

what are we looking for in antibody

Answer 11

-binding to cells and kill them after activation of toxin at antibody binding site
- activate other cells at antibody binding site
- target CD8+ T cells to killed cells
- avidity
-Fc receptor binding
-stability on mucosa/IgA
-absence of effector function
-bi-specific antibody

Question 12

how does T cells kills cells that are affected by virus

Answer 12

T cells kills cells via antibody specificity rather than by TCR

Question 13

what is Intact antibodies

Answer 13

is a full antibody

Question 14

what is a fragment

Answer 14

small part of antibody

Question 15

what is bi specific antibody fragment

Answer 15

is an antibody that cross link two different epitope

Question 16

hat is the benefit of bi specific antibody fragment

Answer 16

it has ability to cross link two different cells: CD8+ cytotoxic T cells and bacteria or cancer cell which induce or promote virus cell killing

Question 17

what is the Systems for production of recombinant antibodies and fragments

Answer 17

• in vitro: no glycosylation
• Escherichia coli : only fragments and no glycosylation
  -Pichia pastoris : only fragments
  insect cells
  myeloma cells
  CHO cells
  COS cells
  HEK293 cells
  animals and plant

Question 18

what are the two important carbohydrate on proteins/antibody

Answer 18

O-linked and N-linked

Question 19

can carbohydrate in antibodies has important function if they are found on variable domain

Answer 19

yes, the specificity

Question 20

what does carbohydrate found on antibody affects

Answer 20

• in constant domain it affects effector function
• in variable domains it affects specificity

Question 21

what is the important aspects of recombinant antibody

Answer 21

is the nature of their carbohydrates